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The molecular characterization of samples from works of art can provide valuable insights into the composition of ancient restoration materials and their conservation state. Here, we present a novel experimental protocol for the molecular characterization of a specific adhesive used in historical pa...
ORGANISM(S): Bos taurus (Bovine) linum usitatissimum Oryctolagus cuniculus (Rabbit) Triticum aestivum (Wheat) Secale cereale Acipenser sturio 
2024-10-17 | PXD051480 | Pride
In order to identify miR-21 targets by a biochemical high-throughput method, we immunopurified RISC Complex and associated mRNAs in both control and miR-21 overexpressing Jurkat cells. Following transduction of miR-21 encoding construct (or a cognate control vector) in Jurkat cells, we used microarr...
ORGANISM(S): Homo sapiens 
T-lymphocyte activation is efficiently mimicked in vitro by treatment with anti CD3 / anti CD28 antibodies. We report miR-21 induction upon CD3/CD28 stimulation of primary T-lymphocytes. In order to assess the function of miR-21 in T-lymphocytes we interfered with miR-21 function by lentiviral trans...
ORGANISM(S): Homo sapiens 
Calcific aortic valve disease (CAVD) is the most common valvular heart disease in older adults, but no medical therapy is currently able to halt or reverse its progression, partly because the molecular mechanisms of valve remodeling remain incompletely understood. Here, we aimed to dissect the extra...
ORGANISM(S): Homo sapiens (Human) 
2026-05-29 | PXD073246 | Pride
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