Sort   by:  
 Page size 
TranscriptomicsCleavage Factor Im as a key regulator of 3’ UTR length
In eukaryotes, the 3' ends of RNA polymerase II-generated transcripts are made in the majority of cases by site-specific endonucleolytic cleavage, followed by the addition of a poly(A) tail. By alternative polyadenylation, a gene can give rise to multiple mRNA isoforms that differ in the length of t...
ORGANISM(S): Homo sapiens 
2012-10-30 | E-GEOD-40137 | biostudies-arrayexpress
TranscriptomicsGenome-wide gene expression analysis in Thoc5- and CFIm68-depleted HeLa cells
We found that CFIm68, a mRNA cleavage and polyadenylation factor implicated for alternative polyadenylation site choice, was co-purified with Thoc5, a component of human THO/TREX. Microarray analysis using human HeLa cells reveals knockdown of Thoc5 affects the expression of a subset of non-heat sho...
ORGANISM(S): Homo sapiens 
2013-05-21 | E-GEOD-42327 | biostudies-arrayexpress
TranscriptomicsGenome?wide analysis of pre?mRNA 3' end processing reveals a decisive role of human cleavage factor I in the regulation of 3' UTR length: A-seq
Through alternative polyadenylation, human mRNAs acquire longer or shorter 3' untranslated regions, the latter typically associated with higher transcript stability and increased protein production. To understand the dynamics of polyadenylation site usage, we mapped transcriptome?wide both binding s...
ORGANISM(S): Homo sapiens 
2012-05-15 | E-GEOD-37037 | biostudies-arrayexpress
ProteomicsMpe1 senses the polyadenylation signal in pre-mRNA to promote efficient cleavage and control polyadenylation
Crosslinking mass spectrometry dataset of the Mpe1 and Cft2 bound to the yeast CPF polymerase module. Sulfo-SDA is the crosslinker used.
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2023-01-12 | PXD027482 | JPOST Repository
TranscriptomicsCFIm25 links alternative polyadenylation to glioblastoma tumour suppression
Purpose: To identify all of the APA targets of CFIm25 on a global scale and develop an algorithm that can idenitify APA events from standard RNA-seq data Methods: RNA from HeLa cells treated with control siRNA and CFIm25 siRNA were subject to RNA-Seq. Using a custom-designed algorithm to mine RNA-s...
ORGANISM(S): Homo sapiens 
2014-05-15 | E-GEOD-42420 | biostudies-arrayexpress
UnknownMutation in cpsf6/CFIm68 (Cleavage and Polyadenylation Specificity Factor Subunit 6) causes short 3'UTRs and disturbs gene expression in developing embryos, as revealed by an analysis of primordial germ cell migration using the medaka mutant naruto.
Not available
S-EPMC5333813 | biostudies-literature
UnknownCellular Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Mediates Nuclear Import of Human Bocavirus 1 NP1 Protein and Modulates Viral Capsid Protein Expression.
Not available
S-EPMC6955249 | biostudies-literature
ProteomicsAlternative polyadenylation factor CPSF6 regulates temperature compensation of the mammalian circadian clock
A defining property of circadian clocks is temperature compensation, characterized by the resilience of circadian free-running periods against changes in environmental temperature.We quantify global changes in 3´UTR length as well as gene and protein expression between wild type and CPSF6 knock-down...
ORGANISM(S): Homo sapiens (Human) 
2023-05-23 | PXD029343 | Pride
Eukaryotic translation initiation factor 2 subunit 1 Integrative proteomics Cleavage and polyadenylation specificity factor subunit 6 Circadian clock Temperature compensation Alternative polyadenylation 
TranscriptomicsCoupling between alternative polyadenylation and alternative splicing is limited to terminal introns
Alternative polyadenylation has been implicated as an important regulator of gene expression. In some cases, alternative polyadenylation is known to couple with alternative splicing to influence last intron removal. However, it is unknown whether alternative polyadenylation events influence alternat...
ORGANISM(S): Homo sapiens 
2016-03-14 | E-GEOD-79157 | biostudies-arrayexpress
UnknownThe Drosophila homologue of the 64 kDa subunit of cleavage stimulation factor interacts with the 77 kDa subunit encoded by the suppressor of forked gene.
Not available
S-EPMC102530 | biostudies-literature
Sort   by:  
 Page size