Sort   by:  
 Page size 
ProteomicsLabel-free comparative data independent (DIA) nanoLC-MS/MS analysis of protein expression profiles of mouse primary dendritic cells (DC) from B6 mice on normal and high fructose/high fat (HFHF) diet
A diet rich in saturated fat and carbohydrates causes a low-grade chronic inflammation in several organs including nonalcoholic steatohepatitis. The environment-driven lipotocicity and glucotoxicity induce tissue damage, which promotes dendritic cell adjuvanticity, and supports an MHC-class-II immun...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-22 | PXD026190 | Pride
Mouse dendritic cells (cd11c+) proteomics  dia/dda nano lc-ms/ms orbitrap fusion tribrid mass spectrometer 
ProteomicsNano LC-MS/MS DDA mass spectrometry profiling of I-Ab MHCII immunopeptidome eluted from B6 mice on normal and high fructose/high fat (HFHF) diet
In Dendritic cells (DC), the MHC II eluted immunopeptidome reflects the antigenic composition of the microenvironment. Proteins are transported and processed into peptides in endosomal MHC II compartments through autophagy or phagocytosis; extracellular peptides can also directly bind MHC II protein...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-22 | PXD026068 | Pride
Mouse i-ab immunopeptidome  dda nanolc-ms/ms  orbitrap fusion tribrid mass spectrometer 
ProteomicsCharacterization of I-Ab MHCII immunopeptidome eluted from control and obese (Ob/Ob) mice (2)
In Dendritic cells (DC), the MHC II eluted immunopeptidome reflects the antigenic composition of the microenvironment. Proteins are transported and processed into peptides in endosomal MHC II compartments through autophagy or phagocytosis; extracellular peptides can also directly bind MHC II protein...
ORGANISM(S): Mus musculus (Mouse) 
2021-03-21 | PXD018783 | Pride
Mouse i-ab mhcii peptidome  lc/ms/ms dda on qe mass spectrometer 
ProteomicsLabel free DIA proteomic analysis of thoracic lymph from cigarette smoke (CS)-exposed mice
The lymphatic vasculature is critical for lung function, but defects in lymphatic functionin the pathogenesis of lung disease is understudied. To further assess the contribution of lymphatics to the pathogenesis of lung emphysema we used a mouse model of cigarette smoke (CS)-induced emphysema, and a...
ORGANISM(S): Mus musculus (Mouse) 
2022-05-10 | PXD031413 | Pride
Label free proteomics  dia proteomics and peptidomics of mouse lymph  dda spectral libraries 
ProteomicsLabel-free quantitative (LFQ) proteomic analysis of redox mediated posttranslational modifications (PTM) in the cartilage from young and old osteoarthritis (OA) mice with or without the senolytic treatment
Senolytic drugs are designed to selectively clear senescent cells (SnCs) that accumulate with injury or aging. In a mouse model of osteoarthritis (OA), senolysis yields a pro-regenerative response. However, recent research studies showed that the therapeutic benefit is reduced in aged mice. Increase...
ORGANISM(S): Mus musculus (Mouse) 
2023-09-26 | PXD031782 | Pride
Mouse model of osteoarthritis (oa)  senolytic treatment  label-free quantitative (lfq) proteomic  analysis of redox ptms in the cartilage proteins 
ProteomicsLabel-free comparative data dependent (DDA) nanoLC-MS/MS analysis of redox mediated posttranslational modifications (PTMs) in the proteomes from mouse primary dendritic cells (DC) isolated from control B6 and obese (Ob/Ob) mice
Proteomic modifications linked to non-enzymatic glycation and glycoxidation are common in all tissues under hyperglycemic conditions, as present in metabolic syndrome, type 2 diabetes (T2D), mice bearing a homozygous mutation in leptin (so-called Ob/Ob mice, a common model of obesity and T2D) and mi...
ORGANISM(S): Mus musculus (Mouse) 
2021-03-21 | PXD024239 | Pride
Control b6 and ob/ob mice dendritic cells Bottom-up proteomics on qehf and velos orbitrap 
ProteomicsLabel-free quantitative data dependent (DDA) nano-LC-MS/MS analysis of proteomic changes in the mesenteric lymph harvested from healthy (control) and DSS-fed mice models of colitis
The gastrointestinal mucosa constitutes a critical barrier where millions of microbes and environmental antigens can interact with the host immune system. Intestinal barrier defects have been linked to a broad range of pathological states, including autoimmune, cancer and neurodegenerative diseases....
ORGANISM(S): Mus musculus (Mouse) 
2024-08-09 | PXD044885 | Pride
Mouse mesenteric lymph  dda nano-lc/ms/ms 
ProteomicsIdentification of the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity in the rat liver.
To identify the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity we purified subcellular organelles including cytosol, late endosomes (LE/MVBs) and lysosomes (CMA+) from the rat liver and performed co-Ip experiment with antibodies directed against the major cellular...
ORGANISM(S): Rattus norvegicus (Rat) 
2024-01-26 | PXD046797 | Pride
Cma vs microautophagy  hsc-70-co-ip-ms/ms in late endosomes Rat liver Lysosomes and cytosol 
ProteomicsLabel-Free Proteomics Profiling of Nanovesicles Isolated from Cultured Salivary Glands Isolated from Partially-Fed Adult Female Ixodes scapularis
Ticks are ectoparasites that have co-evolved with their hosts for millions of years. Unlike other blood-feeders, hard ticks take several days to complete a blood-meal, which makes them especially susceptible to the detection by host immune responses. To overcome this, ticks have developed a vast arr...
ORGANISM(S): Ixodes scapularis (Black-legged tick) (Deer tick) 
2021-06-07 | PXD018779 | Pride
Salivary glands ixodes scapularis  label free proteomics  qexactive nano-lc/ms/ms 
ProteomicsQualitative and label-free quantitative analysis of protein changes in the lymphatic muscle cells isolated in a hind-limb lymphatic injury model in the sheep
We developed a large animal model, with clinically relevant gravitational loading on the lymphatic vasculature, that enables longitudinal tracking of lymphatic function in vivo as well as end-point analysis of vessel function and mechanics. One of two parallel lymphatic vessels in the sheep hind lim...
ORGANISM(S): Ovis aries 
2021-01-12 | PXD014343 | Pride
Sheep lymphatic muscle cells 
Sort   by:  
 Page size