Hypoxia in stem cell niches and inner core of solid tumors contributes to cellular senescence and differentiation inhibition. It directly increases histone methylationby inhibiting the activities of O2-and α-ketoglutarate (α-KG)-dependent histone lysine demethylases. This study is the first to demon...
NoPro-clipping is a method for detecting and quantifying non-proteinaceous ubiquitination from cell lysates and mammalian tissue. The method utilising Ub-clippases to cleave ubiquitin but leave the C-terminal diglycine remnant attached to the protein and non-protein substrates. Following protein exc...
