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Coilin is a scaffold protein essential for the structural integrity of Cajal Bodies, which are non-membranous nuclear organelles that are thought to facilitate assembly and maturation of nuclear RNPs, including spliceosomal snRNPs. To investigate further coilin’s functions in plant cells, and to ide...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-07-27 | PXD027146 | Pride
To investigate location of Cajal bodies (CB) inside the cell nucleus, we created a stable cell line expressing the GFP version of CB-marker protein coilin at endogenous levels and performed ChIP-seq with anti-EGFP antibody. ChIP-seq revealed close association of CBs wih U3 genes, snRNA genes and his...
ORGANISM(S): Homo sapiens 
Our ChIP resuls suggested that coilin association with U3, snRNA and histone genes might be dependent on coilin-RNA interaction. We used iCLIP of coilin-GFP expressed in HeLa and P19 cell lines at endogenous levels to identify coilin RNA targets and investigate RNA-binding specificity. P19 cells exp...
ORGANISM(S): Homo sapiens 
Determining the steady-state levels of proteins in the Arabiopsis coilin mutant plants
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-14 | PXD029502 | Pride
Coilin Mutation in Zebrafish Impairs the Innate Immune Zebrafish
iCLIP mapping of coilin-RNA interactions in HeLa and P19 cells
Coilin is a nuclear protein known for its roles in assembling the Cajal Body and participating in the biogenesis of ribonucleoproteins. We have uncovered novel functions for coilin in promoting microRNA biogenesis and the phosphorylation/SUMOylation of various proteins, through which we have observe...
ORGANISM(S): Danio rerio 
2025-04-30 | GSE273717 | GEO
Our ChIP resuls suggested that coilin association with U3, snRNA and histone genes might be dependent on coilin-RNA interaction. We used iCLIP of coilin-GFP expressed in HeLa and P19 cell lines at endogenous levels to identify coilin RNA targets and investigate RNA-binding specificity. P19 cells exp...
ORGANISM(S): Homo sapiens Mus musculus 
2014-11-06 | GSE58448 | GEO
The cell nucleus contains distinct biomolecular condensates that form at specific genetic loci, organize chromosomes in 3D space, and regulate RNA processing. Among these, Cajal bodies (CBs) require key “scaffolding” proteins for their assembly, which is not fully understood. Here we employ proximit...
ORGANISM(S): Homo sapiens (Human) 
2024-11-10 | PXD042406 | Pride
Arabidopsis COILIN orchestrates transcriptional as well as post-transcriptional regulation to combat pathogens
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