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MetabolomicsNon-targeted in vitro metabolomics for proteome-scale identification of novel enzymes in Escherichia coli
Many yet undiscovered enzymes still limit our understanding of metabolism. Here, we develop a high-throughput mass spectrometry approach to comprehensively profile proteome-scale protein collections for in vitro enzymatic activity. Overexpressed or purified proteins are incubated in a supplemented m...
2016-01-11 | MTBLS373 | MetaboLights
ProteomicsComplete Expressed Proteome of Bartonella Henselae
Using a discovery proteomics approach, the expressed proteome of Bartonella henselae, a Gram-negative prokaryotic model organism was exhaustively studied under two conditions that mimic those encountered in different hosts. Using the analysis-driven experimentation (ADE) feedback-loop strategy, w...
ORGANISM(S): Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) 
2013-07-18 | PXD000153 | Pride
Complete membrane proteome Bartonella henselae Complete proteome Ade 
TranscriptomicsDirected shotgun proteomics guided by RNA-Seq identifies a complete expressed prokaryotic proteome
Prokaryotes are, due to their moderate complexity, particularly amenable to the comprehensive identification of the protein repertoire expressed under different conditions. We applied a generic strategy to identify a complete expressed prokaryotic proteome, which is based on the analysis of RNA and ...
ORGANISM(S): Bartonella henselae 
2013-04-23 | E-GEOD-44564 | biostudies-arrayexpress
ProteomicsNear complete quantitative proteomics for Yeast
In this study, we attempted to achieve to date the most comprehensive coverage of the yeast proteome during log-phase growth and in the K11R mutant ubiquitin yeast strain with a combination of (1) extensive gel molecular weight fractionation and use of either of two digestion enzymes to improve cove...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-08-26 | PXD001928 | Pride
Yeast High coverage Silac Proteome Lc-ms/ms 
TranscriptomicsDirected shotgun proteomics guided by RNA-Seq identifies a complete expressed prokaryotic proteome
Prokaryotes are, due to their moderate complexity, particularly amenable to the comprehensive identification of the protein repertoire expressed under different conditions. We applied a generic strategy to identify a complete expressed prokaryotic proteome, which is based on the analysis of RNA and ...
ORGANISM(S): Bartonella henselae 
2013-04-23 | GSE44564 | GEO
ProteomicsCopy number analysis of the murine platelet proteome spanning the complete abundance range
Knowledge of the identity and quantity of expressed proteins of a cell type is a prerequisite for a complete understanding of its molecular functions. Mass spectrometry (MS)-based proteomics has allowed the identification of the entire protein complement of yeast and the close-to-complete set of pro...
ORGANISM(S): Mus musculus (Mouse) 
2015-08-18 | PXD000747 | Pride
Murine Platelet 
TranscriptomicsTranscription profiling by high throughput sequencing of HeLa human cancer cell line
Illumina single-end sequencing of Hela_2 (HeLa cell line). While the number and identity of proteins expressed in a single human cell type is currently unknown, this fundamental question can be addressed by advanced mass spectrometry (MS)-based proteomics. On-line liquid chromatography coupled to hi...
ORGANISM(S): Homo sapiens 
2011-11-12 | E-MTAB-823 | biostudies-arrayexpress
UnknownThe yeast proteome database (YPD) and Caenorhabditis elegans proteome database (WormPD): comprehensive resources for the organization and comparison of model organism protein information.
Not available
S-EPMC102421 | biostudies-literature
UnknownHuman Proteome Project Mass Spectrometry Data Interpretation Guidelines 3.0.
Not available
S-EPMC6986310 | biostudies-literature
UnknownProgress on Identifying and Characterizing the Human Proteome: 2019 Metrics from the HUPO Human Proteome Project.
Not available
S-EPMC6898754 | biostudies-literature
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