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ProteomicsIn-depth proteome analysis of Hela cell culture conditioned media containing fetal bovine serum.
We performed DIA-MS based proteomic analysis of Hela cells conditioned media containing fetal bovine serum by albumin (Alb)-depletion and non-depletion. In addition, we attempted to enrich secreted proteins by reducing the volume of culture medium (10mL, 5mL and 2mL). The supernatants of each cultur...
ORGANISM(S): Homo Sapiens (human) 
2022-02-22 | PXD024272 | JPOST Repository
ProteomicsHematopoietic stem cell derived conditioned media metabolomics
Metabolomic data of conditioned media derived from culturing hematopoietic stem cells.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-02-14 | MSV000097113 | MassIVE
ProteomicsProteome of human and mouse astrocytes and astrocyte-conditioned medium
Proteome of human and mouse astrocytes and astrocyte-conditioned medium
ORGANISM(S): Homo Sapiens Mus Musculus 
2025-02-05 | PXD060495 |
TranscriptomicsMicroarray Analysis of Genes Regulated by Macrophage-Conditioned Media Treatment of Human Pre-Adipocytes
A major development in the study of obesity is the recognition that the condition is characterised by chronic mild inflammation. Within adipose tissue, this involves the infiltration of macrophages, as well as the direct inflammatory response of the adipocytes and pre-adipocytes. This study has us...
ORGANISM(S): Homo sapiens 
2011-12-21 | E-GEOD-27503 | biostudies-arrayexpress
ProteomicsVariations of secretome profiles according to conditioned medium preparation: the example of human mesenchymal stem cell derived adipocytes
One challenging point in analysing cellular secretome collected as conditioned medium is cross contamination by cell culture media components, especially bovine serum proteins. A common approach for serum removal is to wash the cells, an alternative is to grow cells using serum-free conditions. Give...
ORGANISM(S): Homo sapiens (Human) 
2015-07-03 | PXD002217 | Pride
Adipocyte/conditioned medium/mesenchymal stem cell /secretome 
TranscriptomicsMouse high grade astrocytoma cells (GL261) treated with neural stem/precursor cell (NPC) conditioned medium or non-conditioned medium (control)
Primary astrocytomas of high histopathological grade (HG-astrocytomas) are largely restricted to older patients and are almost invariably fatal despite multimodal therapy. Here, we show that the young brain has an endogenous defense mechanisms against HG-astrocytomas. Neural precursor cells (NPCs) m...
ORGANISM(S): Mus musculus 
2012-04-30 | E-GEOD-37671 | biostudies-arrayexpress
TranscriptomicsUterine stromal cell_cultured with uNK conditioned Medium
The endometrium contains a distinct population of immune cells consisting of 70% natural killer (NK) cells that undergo cyclic changes during the menstrual cycle. However, how these uterine NK (uNK) cells interact with uterine stromal cells (SC) remains unclear. We therefore investigated the paracri...
ORGANISM(S): Homo sapiens 
2010-06-24 | E-GEOD-9718 | biostudies-arrayexpress
GenomicsRNA from mouse lung conditioned medium
RNA from mouse lung conditioned medium
PRJNA675882 | ENA
TranscriptomicsTranscriptional responses to TGFβ1 and conditioned media in mammary epithelial cells
To investigate TGFβ1 (a key member of the TGFβ superfamily) signaling in non-cancerous and cancerous cells derived from breast epithelium and what further response it might generate, we treated the cells with TGFβ1 or with conditioned media. We selected MCF10A non-tumorigenic breast epithelial cells...
ORGANISM(S): Homo sapiens 
2024-12-01 | E-MTAB-13865 | biostudies-arrayexpress
ProteomicsProteomic analysis of extracellular vesicles and conditioned medium from human Adipose-derived Stem/Stromal Cells and Dermal Fibroblasts
Conditioned medium (CM) and extracellular vesicles (EV) from human Adipose-derived Stem/stromal cells (ASC) and Dermal fibroblasts (DF) can represent valid cell substitutes in regenerative medicine and other therapeutic applications. Whether one of the two cell products should be preferred over the ...
ORGANISM(S): Homo sapiens (Human) 
2020-12-23 | PXD020219 | Pride
Adipose-derived stromal cells Label-free quantitative proteomics Extracellular vesicles Dermal fibroblasts Conditioned medium Secretome 
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