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TranscriptomicsSingle cell RNA-seq of isolated human foetal L/M-opsin cone photoreceptor populations labelled using the AAV2/9.pR2.1.GFP reporter
Human cone photoreceptors represent a rare cell population of the human retina and are largely outnumbered by other retinal cell types. Despite their rarity, cone photoreceptors are critical for optimal daylight vision and their loss has the greatest impact on sight during the progression of retinal...
ORGANISM(S): Homo sapiens 
2018-04-20 | E-MTAB-6058 | biostudies-arrayexpress
TranscriptomicsTranscriptome dynamics of developing photoreceptors in 3-D retina cultures recapitulates temporal sequence of human cone and rod differentiation revealing cell surface markers and gene networks
To define molecular mechanisms underlying rod and cone differentiation, we generated H9 human embryonic stem cell line carrying a GFP reporter that is controlled by the promoter of cone-rod homeobox (CRX) gene, the first known marker of post-mitotic photoreceptor precursors. CRXp-GFP reporter in H9 ...
ORGANISM(S): Homo sapiens 
2015-08-03 | E-GEOD-67645 | biostudies-arrayexpress
ProteomicsA mouse photoreceptor proteome resource
To enrich mouse rod and cone photoreceptors for mass spectrometric analysis, we optimized our previously established trituration-based fluorescence-activated cell sorting (FACS) strategy (see Lux et al., 2024 for details; PMID: 38481472). For quantitative mass spectrometry, we utilized an ion mobili...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-08 | PXD066755 | Pride
Label-free protein quantification Mass spectrometry Facs Retina Rod photoreceptors Cone photoreceptors Mpp6/pals2 
TranscriptomicsRNA-seq of isolated human foetal and hiPSC L/M-opsin cone photoreceptor populations labelled using the AAV2/9.pR2.1.GFP reporter
The loss of cone photoreceptor cells, which are critical for optimal daylight vision, have the great impact on vision during retinal degenerations. Retinal differentiation of human induced pluripotent stem cell (hiPSC) sources could provide a renewable source of cone photoreceptors towards developin...
ORGANISM(S): Homo sapiens 
2018-04-20 | E-MTAB-6057 | biostudies-arrayexpress
TranscriptomicsMicroRNA expression profile in mouse cone photoreceptors at P60 [miRNA-seq]
The outer segments of cones serve as light detectors for daylight color vision, and their dysfunction leads to human blindness conditions. We show that the cone-specific disruption of DiGeorge Syndrome Critical Region Gene 8 (DGCR8) in adult mice led to the loss of miRNAs and the loss of outer segme...
ORGANISM(S): Mus musculus 
2014-06-20 | E-GEOD-58500 | biostudies-arrayexpress
GenomicsIdentification of genes with enriched expression in early developing mouse cone photoreceptors
Identification of genes with enriched expression in early developing mouse cone photoreceptors
PRJNA547437 | ENA
TranscriptomicsIdentification of genes with enriched expression in early developing mouse cone photoreceptors
A LHX4 transgenic reporter line with high specificity for developing mouse cone photoreceptors was identified and used to purify early stage cone photoreceptors for profiling by single cell RNA sequencing.
ORGANISM(S): Mus musculus 
2019-06-06 | GSE132272 | GEO
TranscriptomicsRNAseq analysis of Chrnb4-cre driven Dicer conditional knockout (Dicer CKO) whole retinas
We have shown that Chrnb4-cre; Dicerflox/flox retinas (Dicer CKO) display a cone dystrophy by postnatal day P21, independent of rod degeneration. To elucidate which gene pathways were affected by Dicer loss, RNA sequencing was performed in whole neural retinas of P21 control and Dicer CKO mice. This...
ORGANISM(S): Mus musculus 
2019-01-14 | E-MTAB-6133 | biostudies-arrayexpress
TranscriptomicsmiRNAseq analysis of Chrnb4-cre driven Dicer conditional knockout (Dicer CKO) whole retinas
We have shown that Chrnb4-cre; Dicerflox/flox retinas (Dicer CKO) display a cone dystrophy by postnatal day P21, independent of rod degeneration. To elucidate which miRNAs were involved in such phenotype, miRNA sequencing was performed in whole retinas of P21 and 3.5 month old control and Dicer CKO ...
ORGANISM(S): Mus musculus 
2019-01-14 | E-MTAB-6132 | biostudies-arrayexpress
TranscriptomicsPreservation of cone photoreceptors after a rapid yet transient degeneration and remodeling in cone-only Nrl-/- mouse retina
Cone photoreceptors are the primary initiator of visual transduction in the human retina. Dysfunction or death of rod photoreceptors precedes cone loss in many retinal and macular degenerative diseases, suggesting a rod-dependent trophic support for cone survival. Rod differentiation and homeostasis...
ORGANISM(S): Mus musculus 
2011-11-15 | E-GEOD-33674 | biostudies-arrayexpress
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