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MetabolomicsBacteria regulate aging physiology in microalgae via induced vesicle production and the methionine cycle (Metabolomics profiling of FACS purified EVs)

In annually reoccurring patterns, microalgae form blooms that persist and decline thereby contributing massively to global biogeochemical cycles. The decline of blooms is mainly caused by nutrient limitation and goes ahead with the aging of individual algal cells. Nutrient intake can re-initiate ...

2024-05-07 | MTBLS5401 | MetaboLights
ProteomicsProteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations
The metabolic status of individual cells in microbial cultures can differ being relevant for biotechnology, environmental and medical microbiology. However, it is hardly understood in molecular detail due to limitations of current analytical tools. Here, we demonstrate that FACS in combination with ...
ORGANISM(S): Corynebacterium glutamicum ATCC 13032 
2017-11-02 | PXD005812 | Pride
Corynebacterium glutamicum Proteome analysis Facs Label-free quantification Cellular heterogeneity Bcaa producer 
TranscriptomicsExpression data from a new FACS approach isolated hESC derived endoderm cells using transcription factors
We show that high quality microarray gene expression profiles can be obtained following FACS sorting of cells using combinations of transcription factors. We use this transcription factor FACS (tfFACS) methodology to perform a genomic analysis of hESC-derived endodermal lineages marked by combinatio...
ORGANISM(S): Homo sapiens 
2011-08-23 | E-GEOD-24135 | biostudies-arrayexpress
ProteomicsFISH-FACS Proteomics: Enhanced label-free quantitative proteome analysis from ultra-low cell numbers of environmental microorganisms
Metaproteomics is an essential approach to analyse the in situ metabolic activity of microbes across various environments. In such highly diverse environmental samples, the functionality of specific microorganisms of importance often remains underexplored due to the protein inference problem arising...
ORGANISM(S): Polaribacter sp. Aurantivirga 
2025-09-29 | PXD057908 | Pride
Microbial ecology Polaribacter spp. Aurantivirga sp. Marine microorganisms 
ProteomicsComparison of microglial proteomes of MACS and FACS-based purified CD11b+ microglia
Background Proteomic characterization of microglia has been limited by low yield and contamination by non-microglial proteins by magnetic-activated cell sorting (MACS) enrichment strategies. To determine whether a fluorescent-activated cell sorting (FACS)-based strategy overcomes these limitations,...
ORGANISM(S): Mus musculus (Mouse) 
2020-05-11 | PXD015652 | Pride
Mouse acutely purified brain-associated microglia Tmt Macs Facs 
TranscriptomicsTranscription profiling of Zebrafish single cell dissected and FACS sorted embryos to investigate gastrointestinal tract development implicates novel genes and pathways
Zebrafish (Danio rerio) gutGFP transgenic embryos [Tg(XlEef1a1:GFP)s854] were collected at 4 time points: 2 days post fertilization (dpf), 3, dpf, 4 dpf, 6 dpf. Embryos were dissociated into single cells and sorted by FACS based on GFP expression. RNA was extracted from the different cell population...
ORGANISM(S): Danio rerio 
2009-07-19 | E-GEOD-12189 | biostudies-arrayexpress
ProteomicsFACS-Proteomics strategy toward Extracellular Vesicles single-phenotype characterization in biological fluids: insights into the role of Leukocyte-derived EVs in Multiple Sclerosis
The isolation and proteomics characterization of Extracellular Vesicles (EVs) from body fluids represents a stimulating challenge to improve our knowledge of biology, considering the vast heterogeneity in terms of phenotype in vivo. Within a complex and dynamic scenario of EVs isolation and characte...
ORGANISM(S): Homo sapiens (Human) 
2025-05-26 | PXD049036 | Pride
Leukocyte-derived evs Multiple sclerosis Facs-proteomics Tears 
UnknownContinuous FACS sorting of double emulsion picoreactors with a 3D printed vertical mixer.
Not available
S-EPMC11952560 | biostudies-literature
UnknownFACS-Sortable Triple Emulsion Picoreactors for Screening Reactions in Biphasic Environments.
Not available
S-EPMC12360416 | biostudies-literature
UnknownContinuous FACS Sorting of Double Emulsion Picoreactors with a 3D-Printed Vertical Mixer.
Not available
S-EPMC12268830 | biostudies-literature
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