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The isolation and proteomics characterization of Extracellular Vesicles (EVs) from body fluids represents a stimulating challenge to improve our knowledge of biology, considering the vast heterogeneity in terms of phenotype in vivo. Within a complex and dynamic scenario of EVs isolation and characte...
ORGANISM(S): Homo sapiens (Human) 
2025-05-26 | PXD049036 | Pride
The metabolic status of individual cells in microbial cultures can differ being relevant for biotechnology, environmental and medical microbiology. However, it is hardly understood in molecular detail due to limitations of current analytical tools. Here, we demonstrate that FACS in combination with ...
ORGANISM(S): Corynebacterium glutamicum ATCC 13032 
2017-11-02 | PXD005812 | Pride
Metaproteomics is an essential approach to analyse the in situ metabolic activity of microbes across various environments. In such highly diverse environmental samples, the functionality of specific microorganisms of importance often remains underexplored due to the protein inference problem arising...
ORGANISM(S): Polaribacter sp. Aurantivirga 
2025-09-29 | PXD057908 | Pride
Label-free expression proteomics analysis of FACS-sorted mouse podocytes versus non-podocytes
ORGANISM(S): Mus musculus (Mouse) 
2018-12-14 | PXD010601 | Pride
Single-cell proteomics of human bone marrow, FACS panel 2
Large-scale single-cell analyses are of fundamental importance in order to capture biological heterogeneity within complex cell systems, but have largely been limited to RNA-based technologies. Here we present a comprehensive benchmarked experimental and computational workflow, which establishes glo...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-10-21 | MSV000090570 | MassIVE
The widespread adoption of single-cell proteomics (SCP) in biology has been limited by complex workflows and reliance on specialized instrumentation. Here we present EasySCP, a high-throughput and lossless method that integrates FACS-based single-cell sorting, an all-in-one, single-step digestion pr...
ORGANISM(S): Homo Sapiens Mus Musculus 
2025-10-30 | PXD070083 |
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