To study cancer cells heterogeneity at the single cell level we grew cancer cells as spheroids and extracted their RNA preform SmartSeq3xpress. We grew MDA-MB-231 cells on agar coated plates for 5-10 days in DMEM 10% FBS. The spheroids were incubated for 2 hours with Calcein AM and Vybrant Dye 10...
ORGANISM(S): Homo sapiens
