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TranscriptomicsCo-regulation analysis of CP190 and CG9740 [expression]
Gene expression in S2 cells after CG9740 or CP190 RNAi Two biological replicates for each RNAi condition are analyzed including LacZ RNAi as control.
ORGANISM(S): Drosophila melanogaster 
2014-04-18 | E-GEOD-47557 | biostudies-arrayexpress
GenomicsIbf contributes to CP190 recruitment to chromatin [ChIP-seq]
ChIP-Seq peak calling of CP190 in wild-type and Ibf2 mutant Drosophila melanogaster third instar larvae Two wild-type and two Ibf2 mutant Drosophila melanogaster third instar larvae were sequenced.
ORGANISM(S): Drosophila melanogaster 
2014-04-18 | E-GEOD-52240 | biostudies-arrayexpress
GenomicsRNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function
Chromatin insulators and Polycomb group (PcG) complexes control nuclear organization to effect changes in gene expression. In Drosophila, RNA silencing pathways influence long range interactions mediated by PcG proteins and nuclear localization of the gypsy insulator; however, the underlying mechan...
ORGANISM(S): Drosophila melanogaster 
2011-08-15 | E-GEOD-22623 | biostudies-arrayexpress
GenomicsIbf1 and Ibf2 are DNA-binding proteins required for insulator function in Drosophila [ChIP-seq]
Here we report on the identification of two previously uncharacterized proteins as CP190 interacting proteins, that we have named Ibf1 and Ibf2. These proteins localize at insulator bodies and associate with chromatin at CP190-binding sites throughout the genome. We also show that Ibf1 and Ibf2 are ...
ORGANISM(S): Drosophila melanogaster 
2014-04-18 | E-GEOD-46614 | biostudies-arrayexpress
TranscriptomicsAnalysis of genome-wide binding of the insulator factors CTCF and CP190
We identified about 3000 CTCF sites in the genome. In addition to binding to CTCF sites we found CP190 to bind to transcriptional start sites of actively transcribed genes where it inversely correlates with nucleosome occupancy. Keywords: ChIP-chip CTCF and CP190 ChIP. 2 biological replicates per e...
ORGANISM(S): Drosophila melanogaster 
2010-05-05 | E-GEOD-12749 | biostudies-arrayexpress
TranscriptomicsKc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown
This SuperSeries is composed of the following subset Series: GSE30686: Gene expression analysis of Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown GSE30740: Distribution of Drosophila insulator proteins after ecdysone treatment in Kc cells Refer to individual Seri...
ORGANISM(S): Drosophila melanogaster 
2011-10-13 | E-GEOD-30741 | biostudies-arrayexpress
TranscriptomicsGene expression analysis of Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown
This study examines the changes in genes expression that occur in Drosophila melanogaster during the ecdysone response as well as during RNAi knockdown of the insulator protein, CP190. Analysis was performed in Kc cells after 0, 3, and 48 hours of ecdysone treatment in the presence of either contro...
ORGANISM(S): Drosophila melanogaster 
2011-10-13 | E-GEOD-30686 | biostudies-arrayexpress
ProteomicsM1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity
In order to identify more gypsy insulator interactors, we performed immunoaffinity purification of Drosophila embryonic nuclear extracts using antibodies specific for CP190 and analyzed the eluates using quantitative mass spectrometry.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-06-07 | PXD026493 | Pride
Mass spectrometry Drosophila Cp190 Embryonic nuclear extract 
ProteomicsEssential role of Cp190 in physical and regulatory boundary formation in Drosophila
Animal genomes fold into contact domains defined by enhanced internal contact frequencies with debated functions in establishing independent gene regulatory domains. A large fraction of contact domains in mammals are formed by stalling of chromosomal loop-extruding cohesin by CTCF at domain boundari...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-05-30 | PXD027795 | Pride
Genome architecture Development Drosophila 
TranscriptomicsTranscription profiling of Drosophila homozygous CP190[1] 0 hour white prepupae (non tubby, red malpighian tubules) homozygous CP190[2] 0 hour white prepupae (non tubby, red malpighian tubules) homozygous red[1] e[1] 0 h white prepupae (source from which the mutated chromosome was obtained)
CP190 is a Drosophila protein of unknown function that exhibits cell cycle-specific localisation to the centrosome during mitosis and the nucleus during the interphase. CP190 associates with multiple sites on polytene chromosomes and its predicted amino acid sequence identifies four C2h2 zinc finger...
ORGANISM(S): Drosophila melanogaster 
2004-04-21 | E-FLYC-1 | biostudies-arrayexpress
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