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ProteomicsExtracellular vesicles isolated from fresh vs frozen melanoma tissues are similar in purity, protein composition, and tumor antigenicity
Extracellular vesicles (EVs) are nano-sized particles released by cells, enriched in disease-related molecules, gaining attention as potential tumor biomarkers and useful tools for cancer immunotherapy. Tumor tissues are valuable sources of EVs, reflecting the tumor's molecular profile. This study i...
ORGANISM(S): Homo sapiens (Human) 
2026-06-01 | PXD068552 | Pride
Tmt Extracellular vesicles Melanoma Isolation Global quantitative proteomics 
ProteomicsHeat inactivation of foetal bovine serum performed after EV-depletion influences the proteome of cell-derived extracellular vesicles
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be influenced by cell culture conditions such as the presence of foetal bovine serum (FBS). Although several studies have evaluated the effect of removing FBS-derived EVs by ultracentrifugation (UC), le...
ORGANISM(S): Bos taurus (Bovine) 
2024-01-26 | PXD047505 | Pride
Ev depletion by ultracentrifugation Foetal bovine serum (fbs) Extracellular vesicles Fbs heat-inactivation 
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