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ProteomicsStructural requirements for photo-induced RNA-protein cross-linking
Cross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) was used to study the UV cross-linking behavior of in vitro reconstituted FOX1 RRM in complex with its cognate RNA sequence, the Fox binding element (FBE), (U)GCAUGU. The FBE heptanucleotide was subsequently mutated, and ...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD031381 | Pride
Uv cross-linking  mbnl1  fox1  ptbp1  sf3a1  u1  u2  cross-linking  xl-ms  structural proteomics  protein-rna  rna-protein  protein-rna complex  rna-protein complex  clir-ms 
ProteomicsComparison of OpenPepXL and xQuest on a Labeled Cross-Linking MS dataset
These data sets are part of a large study aimed at the comparison of experimental and computational workflows used in chemical cross-linking coupled to mass spectrometry. Bovine serum albumin (BSA) was used as a model protein. Two different cross-linking chemistries were used: disuccinimidyl suberat...
ORGANISM(S): Bos taurus (Bovine) 
2020-10-20 | PXD014523 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  bovine serum albumin 
ProteomicsIn culture cross-linking of bacterial cells reveals large scale dynamic protein-protein interactions at the peptide level.
Identification of dynamic protein-protein interactions at the peptide level on a proteomic scale is a challenging approach that is still in its infancy. We have developed a system to cross-link cells directly in culture with the special lysine cross-linker bis(succinimidyl)-3-azidomethyl-glutarate (...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2017-05-23 | PXD006287 | Pride
Escherichia coli Bacillus subtilis Rna polymerase Membrane permeability Transcription. Bamg (bis(succinimidyl)-3-azidomethyl-glutarate) In vivo cross-linking Translation 
TranscriptomicsPTBP1 - Modified iCLIP
This experiments was performed in HeLa cells according to the iCLIP protocol with the following modifications: no antiRNase was used and the concentration of RNase I was 0.5 U/ml. In iCLIP4, the dephosphorylation step was omitted from the standard protocol. The rest of the protocol was identical to...
ORGANISM(S): Homo sapiens 
2016-11-24 | E-MTAB-5026 | biostudies-arrayexpress
TranscriptomicsCross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor
A complex programme of regulation governs gene expression during development of the morphologically and biochemically complex eubacterial genus Streptomyces. Earlier work has suggested a model in which 'higher level' pleiotropic regulators activate 'pathway-specific' regulators located within chromo...
ORGANISM(S): Streptomyces coelicolor 
2006-02-01 | E-GEOD-4138 | biostudies-arrayexpress
UnknownTraffic-Related Air Pollution, Noise at School, and Behavioral Problems in Barcelona Schoolchildren: A Cross-Sectional Study.
Not available
S-EPMC4829987 | biostudies-literature
TranscriptomicsDietary folate depletion and repletion in A/J and C57BL/6J mice
Defects in homocysteine and folate metabolism are associated with increased risks for neural tube and congenital heart defects, cardiovascular disease and stroke, cancers, and neurodegeneration. In many but not all cases, dietary supplementation with folate significantly reduces the severity and in...
ORGANISM(S): Mus musculus 
2008-08-27 | E-GEOD-9242 | biostudies-arrayexpress
TranscriptomicsThe RNA-binding protein Musashi1 is a central regulator of adhesion pathways in glioblastoma
The conserved RNA-binding protein Musashi1 (MSI1) has emerged as a key oncogenic factor in numerous solid tumors, including glioblastoma. However, its mechanism of action has not yet been established comprehensively. We set out to map its impact on the transcriptome in U251 cells using RNA-seq and i...
ORGANISM(S): Homo sapiens 
2015-06-29 | E-GEOD-68800 | biostudies-arrayexpress
TranscriptomicsResponse of tobacco cells to Glucan elicitor
We have previously reported that the responses of tobacco tissues treated with AaGlucan are similar to those for laminarin [1]. To better understand the overall responses and signaling pathways in tobacco, we have identified here AaGlucan and laminarin early response genes, using a custom 16K BY-2 m...
ORGANISM(S): Nicotiana tabacum 
2007-10-16 | E-GEOD-6948 | biostudies-arrayexpress
UnknownLysyl oxidase-like 2 (LOXL2)-mediated cross-linking of tropoelastin.
Not available
S-EPMC6629125 | biostudies-literature
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