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TranscriptomicsMeasuring transcriptional differences between Caulobacter crescentus cultured in nitrate versus ammonium as the sole nitrogen source
This study is measuring the steady-state levels of mRNA in wild-type Caulobacter crescentus grown in M2 defined medium containing either ammonium or nitrate as the sole nitrogen source. Four independent cultures of Caulobacter crecentus were grown in each of two medium conditions: M2(nitrate)glucose...
ORGANISM(S): Caulobacter vibrioides 
2010-06-20 | E-GEOD-21203 | biostudies-arrayexpress
TranscriptomicsTesting the effects of nitric oxide (oxidative) stress on Caulobacter transcription
The goal of this study was to measure the effects of nitric oxide exposure (using DETA NONOate as a nitric oxide donor) on transcription in Caulobacter. Untreated Caulobacter crescentus were grown to a density of 0.3 (at OD660) in PYE medium (pH 7) in rolled culture tubes. DETA-NONOate treated Cau...
ORGANISM(S): Caulobacter vibrioides 
2010-06-20 | E-GEOD-21139 | biostudies-arrayexpress
TranscriptomicsMeasuring the effects of hypoxia on transcription in Caulobacter crescentus
To test the effects of hypoxia on transcription in Caulobacter crescentus, we cultured cells in a New Brunswick bioreactor under controlled conditions. Prior to innoculation, the medium was bubbled with laboratory air at maximum flow and stirred at 300 rpm for 2 hours. After this period, the mediu...
ORGANISM(S): Caulobacter vibrioides 
2010-06-21 | E-GEOD-21127 | biostudies-arrayexpress
ProteomicsCaulobacter crescentus GsrN mutants proteome analysis
In order to elucidate the global regulatory effects of the sRNA, GsrN, in Caulobacter crescentus, we submitted soluble lysates from a gsrN knockout and over-expression stain for analysis, as well as, a wild-type sample for LC-MS/MS
ORGANISM(S): Caulobacter crescentus CB15 
2018-03-26 | PXD008128 | Pride
Caulobacter crescentus gsrn srna 
ProteomicsCaulobacter crescentus GsrN overexpression proteome during hyperosmotic stress
In order to better define the global regulatory effects of the sRNA, GsrN, in Caulobacter crescentus during hyperosmotic stress, we submitted soluble lysates from a gsrN over-expression stain , as well as, a wild-type sample for LC-MS/MS analysis.
ORGANISM(S): Caulobacter crescentus CB15 
2019-11-12 | PXD010072 | Pride
Caulobacter crescentus Sucrose Srna overexpression Hyperosmotic stress 
TranscriptomicsThe Brucella abortus virulence regulator, LovhK, is a sensor kinase in the general stress response signaling pathway
We sequenced the transcriptomes of B. abortus 2308 wild type, rpoE1, and lovhK deletion strains to assess gene expression in response to oxidative stress. The data represent 2 independent biological replicates of each strain. Illumina RNA-seq of total mRNA isolated from wild type, rpoE1, and lovhK d...
ORGANISM(S): Brucella melitensis 
2014-09-25 | E-GEOD-59513 | biostudies-arrayexpress
MetabolomicsNon-redundant cardiolipin synthase family proteins shape lipid composition and stress resilience in Bacteroides fragilis

Gut-resident bacteria must withstand membrane-disrupting stresses such as bile acids to persist in the intestinal environment. The Bacteroidota are a dominant phylum of the human gut microbiota. However, the genes responsible for synthesis of membrane lipids in this phylum remain relatively uncha...

2026-10-06 | MTBLS11891 | MetaboLights
TranscriptomicsComparing the transcriptional profiles of a Caulobacter crescentus phyR null mutant to a xylose-inducible phyR overexpression strain
phyR encodes a general stress regulator protein that is conserved among several alpha-proteobacteria. This experiment is quantifying transcription in two Caulobacter strains: one strain is overexpressing phyR (gene CC3477); the other carries a phyR in-frame deletion. Both strains were cultured in ...
ORGANISM(S): Caulobacter vibrioides 
2012-05-31 | E-GEOD-21205 | biostudies-arrayexpress
TranscriptomicsCaulobacter vibrioides CB15N mRNA expression in M2-Glucose Vs. M2-Inositol
The purpose of this experiment was to examine the differential transcriptional profiles of Caulobacter CB15N grown in M2-Glucose versus M2-Inositol. mRNA expression was compared between Caulobacter CB15N cells grown in M2-Glucose vs. M2-Inositol. Four arrays were run, two of each dye combination.
ORGANISM(S): Caulobacter vibrioides 
2010-06-30 | E-GEOD-12414 | biostudies-arrayexpress
ProteomicsUntargeted UHPLC-MS/MS screen of human gut microbiome commensal isolate bacteria from healthy human donor feces and validated standards from 'Microbially-catalyzed conjugation of GABA and tyramine to bile acids'
Untargeted UPLC-MS/MS data from a screen of human gut microbiome commensal isolate bacteria from healthy human donor feces. Isolates include Mediterraneibacter gnavus MSK15.77 (NCBI accession NZ_JAAIRR010000000), Bacteroides ovatus MSK22.29 (NCBI accession NZ_JAHOCX010000000), Bifidobacterium longum...
ORGANISM(S): Human Gut Microbiome Isolate Bacteria 
2023-10-04 | MSV000093035 | MassIVE
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