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An untargeted metabolomics approach using UPLC-ESI-QTOF-MS was performed to explore the metabolome of iceberg lettuce and the changes related to storage time and genetics. Two cultivars with different browning susceptibility, fast- browning (FB) and slow-browning (SB) were studied juts after cutting...
2017-08-03 | MTBLS343 | MetaboLights
Cut-out switch model Membrane identity and GTPase cascades regulated by toggle and cut-out switches Perla Del Conte-Zerial, Lutz Brusch, Jochen C Rink, Claudio Collinet, Yannis Kalaidzidis, Marino Zerial, and Andreas Deutsch: ...
2024-09-02 | BIOMD0000000174 | BioModels
Understanding how gene regulators bind DNA and interact with one another is essential for understanding gene expression, yet existing technologies typically measure either chromatin occupancy or protein–protein interactions in isolation, require high cell numbers, and/or depend on genetic engineerin...
ORGANISM(S): Homo sapiens (Human) 
2026-10-05 | PXD071610 | Pride
CUT&Tag was performed to identify the effect of DIS3 depletion on DNA:RNA hybrids genome-wide. DIS3-AID HCT116 cells were treated with either Auxin (for rapid depletion of DIS3) or DMSO (control). CUT&Tag was performed targeting DNA:RNA hybrids using the CUT&Tag-IT® R-loop Assay Kit (Active Motive) ...
ORGANISM(S): Homo sapiens 
CUT&Tag was performed to identify the effect of DIS3 depletion on RNAPII in response to UV irradiation. DIS3-AID HCT116 cells were treated with either Auxin (for rapid depletion of DIS3) or DMSO (control) before irradiation with 8 J/m2 UVC. CUT&Tag was performed targeting RNAPII-Ser2p or RNAPII-Ser5...
ORGANISM(S): Homo sapiens 
In our attempts to profile different regulators of the WNT/b-catenin transcriptional complex, CUT&RUN failed to produce consistent binding patterns of the non-DNA-binding b-catenin. We developed a modified CUT&RUN protocol, which we refer to as LoV-U (Low Volume and Urea), that enables the generatio...
ORGANISM(S): Mus musculus 
CUT&Tag for H3K27Ac; Colorectal cancer patient derived organoids, HCT26-1T; drug experiment of control vs GSK126.
ORGANISM(S): Homo sapiens 
The goal of this study is to investigate the biological role of the podocyte-specific long non-coding RNA Wt1os (human ortholog WT1-AS) and its contribution to glomerular health and disease. The experimental workflow integrates both in vivo and cell culture approaches. We generated a Wt1os loss-of-f...
ORGANISM(S): Mus musculus 
Here we used laser cutting microdissection and RNA amplification to profile the gene expression in wildtype female germaria and male apex of the testes. These tissue contain germline stem cells and early dividing germ cells. Our goal was to identify genes expressed in these cell types. A direct mi...
ORGANISM(S): Drosophila melanogaster 
The objective was to identify downstream gene targets of pSmad1/5 and pStat3 using the CUT & TAG technique
ORGANISM(S): Danio rerio 
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