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TranscriptomicsSingle-cell transcriptional analysis reveals innate lymphoid cell (ILC)-like cells in zebrafish
Transcriptome data from zebrafish single and bulk cells from blood in five organs. Blood cells were collected from adult Tg(cd4-1:mCherry), Tg(lck:EGFP), Tg(mhc2dab:GFP, cd45:dsRed), AB.
ORGANISM(S): Danio rerio 
2018-11-16 | E-MTAB-7117 | biostudies-arrayexpress
TranscriptomicsSingle-cell transcriptional analysis reveals ILC-like cells in zebrafish
Transcriptome data from zebrafish single cells from guts from either from Tg(lck:EGFP) rag1-/-mutant or wild-type zebrafish were isolated and single cell suspensions were prepared as described in protocol section. Three zebrafish, per each condition (i.e. zebrafish intraperitoneally injected with PB...
ORGANISM(S): Danio rerio 
2018-11-16 | E-MTAB-7159 | biostudies-arrayexpress
GenomicsGenome-wide analysis of GATA1, GATA2, RUNX1, FLI1 and SCL binding in primary human megakaryocytes
We have mapped the binding sites for the five key regulators GATA1, GATA2, RUNX1, FLI1 and TAL1/SCL in primary human megakaryocytes. Statistical analysis identified subsets of enriched as well as depleted combinatorial binding patterns. In particular simultaneous binding by all 5 factors was highly ...
ORGANISM(S): Homo sapiens 
2011-06-01 | E-GEOD-24674 | biostudies-arrayexpress
TranscriptomicsIntegrative single-cell RNA-Seq and ATAC-Seq analysis of human foetal liver and bone marrow haematopoiesis (scRNA-Seq)
This is plate-based (SmartSeq2-Seq) single-cell transcriptome data set from 15 human foetal livers and bone marrow (17-22 PCW). It includes 5,867 cells with an average of 3,074 genes per cell before QC and 4,504 cells with an average of 3,642 genes per cell after QC.
ORGANISM(S): Homo sapiens 
2020-05-25 | E-MTAB-9067 | biostudies-arrayexpress
TranscriptomicsAnalysis of single CD41 cell transcriptomes from Zebrafish
Transcriptome data from individual CD41 expressing cells isolated from kidney and heart of zebrafish. CD41 is a marker both for HSCs and thrombocytes, and with this data we study thrombocyte development as a continuous process. Single cell transcriptomes are matched with flourescence of a CD41:GFP r...
ORGANISM(S): Danio rerio 
2016-01-08 | E-MTAB-3947 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA-Seq data of zebrafish blood cells
Transcriptome data from zebrafish single and bulk cells from blood and testes. Blood cells were collected from adult Tg(cd4:mCherry), Tg(cd41:EGFP), Tg(gata1a:GFP), Tg(lyz:DsRed2), Tg(mfap4:tdTomato), Tg(mpx:EGFP), Tg(runx1:mCherry), Tg(tal1:EGFP) and Tubingen Long Fin wild type fish.
ORGANISM(S): Danio rerio 
2017-11-17 | E-MTAB-5530 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA-sequencing of Spike-ins and mESC using Smart-Seq2 on C1 System
In this study, we assess technical differences between commonly used single-cell RNA-Sequencing (scRNA-Seq) methods. We perform scRNA-seq on a homogenous population of mouse embryonic stem cells along with two kinds of control spike-in molecules to assess sensitivity and accuracy of these specific m...
ORGANISM(S): Mus musculus 
2017-03-29 | E-MTAB-5485 | biostudies-arrayexpress
TranscriptomicsSpatial transcriptomics (10x Visium) of human NSCLC lesions and non-involved tissue
We performed 10X Visium on consecutive 10 µm sections from 8 human NSCLC tumours (total 20 sections), 8 adjacent non-involved lung tissues (total 16 sections) and two healthy donors (total 4 sections)
ORGANISM(S): Homo sapiens 
2024-04-29 | E-MTAB-13530 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA sequencing of healthy and trisomy 21 (Down's syndrome) foetal liver and foetal bone marrow
Here, we performed single cell RNA sequencing (scRNA-seq) of human fetal liver and bone marrow tissue samples from 15 trisomy 21 (Ts21) and 3 healthy foetuses (median age 14 post-conception weeks). The data set is composed of approximately 1.1 million cells from three different populations: CD235- (...
ORGANISM(S): Homo sapiens 
2024-07-22 | E-MTAB-13067 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA-seq atlas of human NSCLC lesions and non-involved tissue
We performed single cell RNA sequencing (scRNA-seq) of NSCLC tumours and matched, adjacent, non-involved lung tissue from 24 patients. The data set is composed of approximately 900,000 cells from two different populations: CD235- (haematopoietic and non-haematopoietic cells depleted of erythrocytes)...
ORGANISM(S): Homo sapiens 
2024-04-29 | E-MTAB-13526 | biostudies-arrayexpress
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