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MetabolomicsMechanism of Hepatotoxicity Induced by Ethanol Extract of Emilia sonchifolia (L.) DC Revealed by Proteomics and Metabolomics

Objective: Hepatotoxicity has been reported for Emilia sonchifolia (L.) DC (Emilia sonchifolia). The plant material is typically prepared using two extraction methods for practical application: water extraction and ethanol extraction. However, our previous research only investigated its wate...

2025-10-30 | MTBLS13237 | MetaboLights
TranscriptomicsKnockdown of SR protein family in P19 cells and RNA-seq of cytoplasmic and whole cell polyA+ RNA
Our data suggest that all SR proteins contribute to mRNA export via NXF1. To identify endogenous export targets we depleted all seven SR proteins individually from P19 WT cells prepared cytoplasmic fractions. We sequenced the cytoplasmic fraction and as a control whole celll RNA from the identical s...
ORGANISM(S): Mus musculus 
2016-03-07 | E-GEOD-69733 | biostudies-arrayexpress
TranscriptomicsIdentification of re-capping substrates for the cytoplasmic capping enzyme complex
The analysis of capped RNAs by massively parallel sequencing has identified a large number of previously unknown transcripts, some of which are small RNAs and others are 5M-bM-^@M-^Y truncated forms of RefSeq genes. The latter may be generated by endonuclease cleavage or by stalling of Xrn1 at defi...
ORGANISM(S): Homo sapiens 
2012-12-20 | E-GEOD-36729 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human U927 cells polysomal and cytoplasmic RNA before and after PMA stimulation
Human histomonocytic U937 cells exhibit macrophage-like properties after phorbol ester stimulation. Accumulating evidence demonstrated that remarkable number of transcripts changed in their amount at total RNA levels. However, post-transcriptional regulation has not been fully elucidated in this m...
ORGANISM(S): Homo sapiens 
2008-06-11 | E-GEOD-1783 | biostudies-arrayexpress
ProteomicsHuman Angiogenin Cytoplasmic Interactomics
Human Angiogenin (hAng) is a member of the ribonuclease A superfamily and a potent inducer of neovascularization. Herein, protein interactions of hAng in the nucleus and cytoplasm of the human umbilical vein cell line EA.hy926, have been investigated by mass spectroscopy. The first gel-free analysis...
ORGANISM(S): Homo sapiens (Human) 
2017-08-11 | PXD006583 | Pride
Immunoprecipation Angiogenin Lc-ms/ms 
TranscriptomicsEfficient cellular fractionation of RNA
The aim of this experiment was to develop new methods to extract pure fractions of nuclear and cytoplasmic RNA. We compared the results of nuclear and cytoplasmic RNA-sequencing to results of the total and polyA+ RNA-sequencing. Cytoplasmic, nuclear, total and polyA+ RNA was extracted from two human...
ORGANISM(S): Homo sapiens 
2013-10-22 | E-MTAB-1898 | biostudies-arrayexpress
TranscriptomicsRNAi knock down of dU2AF50 in Drosophila mel2 cells and profiling of nuclear and cytoplasmic fractions
Nuclear and cytoplasmic RNA were extracted by hypotonic cell lysis from D.-mel2 cell knock down by RNAi against dU2AF50 or with non-specific dsRNA.
ORGANISM(S): Drosophila melanogaster 
2007-07-05 | E-GEOD-1344 | biostudies-arrayexpress
TranscriptomicsADAM13 knockdown in Xenopus laevis cranial neural crest
ADAMs are transmembrane metalloproteases that control cell behavior by cleaving both cell adhesion and signaling molecules. The cytoplasmic domain of ADAMs can regulate the proteolytic activity by controlling the subcellular localization and/or the activation of the protease domain. Here we show tha...
ORGANISM(S): Xenopus laevis 
2011-02-22 | E-GEOD-21517 | biostudies-arrayexpress
ProteomicsMetallosphaera cuprina cultivation on sulfur or yeast extract
By using heterotrophic proteome as reference, 169 proteins were found to change their abundance during autotrophic growth. The up-regulated proteins indicated that M. cuprina fixed CO2 through the previously identified 3-HB-CoA/4-HB-CoA cycle and obtained energy by oxidation of elemental sulfur as e...
ORGANISM(S): Metallosphaera 
2022-02-22 | PXD000676 | Pride
Lc-ms analysis 
ProteomicsAntifungal Synergy: Mechanistic Insights into the R-1-R Peptide and Bidens pilosa Extract as Potent Therapeutics against Candida spp. through Proteomics
Previous work has reported that the peptide derived from LfcinB, R-1-R exhibited anti-Candida activity, which is enhanced when combined with an extract from the Bidens pilosa plant. However, the mechanism of action has not been studied until now. In the current study, an approach to the mechanism of...
ORGANISM(S): Candida albicans (Yeast) 
2024-10-17 | PXD053558 | Pride
Bovine lactoferricin peptides  bidens pilosa  candida albicans Proteomics Antifungal resistance. Disfunction mitochondrial Ros 
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