Sort   by:  
 Page size 
TranscriptomicsProbing nucleosome function: A highly versatile library of synthetic histone H3 and H4 mutants
The structural integrity of the nucleosome is central to regulation of DNA metabolism and transcription. We describe a library of 486 systematic histone H3 and H4 substitution and deletion mutants in Saccharomyces cerevisiae that probe the contribution of each residue to nucleosome function and can ...
ORGANISM(S): Saccharomyces cerevisiae 
2008-09-18 | E-GEOD-10860 | biostudies-arrayexpress
TranscriptomicsExpression profiles of histone point mutants
Accurate chromosome segregation requires that sister kinetochores biorient and attach to microtubules from opposite poles. Kinetochore biorientation relies on the underlying centromeric chromatin, which provides a platform to assemble the kinetochore and to recruit the regulatory factors that ensur...
ORGANISM(S): Saccharomyces cerevisiae 
2013-12-04 | E-GEOD-39903 | biostudies-arrayexpress
ProteomicsGwAAP: A Genome-wide Amino Acid coding-decoding quantitative Proteomic system
GwAAP: A Genome-wide Amino Acid coding-decoding quantitative Proteomic system was designed in which each protein could be assigned with a distinct code. Using synthetic biology technology, the amino acids codes were coded in N terminal of the corresponding proteins in the genome of yeast (Saccharomy...
ORGANISM(S): Saccharomyces Cerevisiae 
2021-12-22 | PXD023100 |
ProteomicsThe de novo design and synthesis of yeast chromosome XIII facilitates investigations on aging
In the era of synthetic biology, design, construction, and utilization of synthetic chromosomes with unique features provide a new strategy to study complex cellular processes such as aging. Herein, we successfully constructed the 884 Kb synXIII of Saccharomyces cerevisiae to investigate replicative...
ORGANISM(S): Saccharomyces Cerevisiae 
2024-10-30 | PXD057330 |
MetabolomicsDeep functional analysis of synII, a 770 kb synthetic yeast chromosome
Here we report the design, construction and characterization of a 770 Kb synthetic yeast chromosome II (synII). With the UPLC-MS method, we demonstrated synII maintains a nearly wild-type phenotype and fitness despite hundreds of designer editing to it. Our phenomics, transcriptomics, proteomics dat...
2016-10-13 | MTBLS296 | MetaboLights
Sort   by:  
 Page size