Metabolomics

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Deep functional analysis of synII, a 770 kb synthetic yeast chromosome

ABSTRACT: Here we report the design, construction and characterization of a 770 Kb synthetic yeast chromosome II (synII). With the UPLC-MS method, we demonstrated synII maintains a nearly wild-type phenotype and fitness despite hundreds of designer editing to it. Our phenomics, transcriptomics, proteomics data futher supports the identity of synII and wild one.

OTHER RELATED OMICS DATASETS IN: PRJNA352710PXD003435

INSTRUMENT(S): Xevo QTof MS (Waters)

PROVIDER: MTBLS296 | MetaboLights | 2016-10-13

REPOSITORIES: MetaboLights

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20150526_Yeast_lipidomic_01_001.raw.zip Raw
20150526_Yeast_lipidomic_01_001_neg.raw.zip Raw
20150526_Yeast_lipidomic_01_002.raw.zip Raw
20150526_Yeast_lipidomic_01_002_neg.raw.zip Raw
20150526_Yeast_lipidomic_02_001.raw.zip Raw
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Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome.

Shen Yue Y   Wang Yun Y   Chen Tai T   Gao Feng F   Gong Jianhui J   Abramczyk Dariusz D   Walker Roy R   Zhao Hongcui H   Chen Shihong S   Liu Wei W   Luo Yisha Y   Müller Carolin A CA   Paul-Dubois-Taine Adrien A   Alver Bonnie B   Stracquadanio Giovanni G   Mitchell Leslie A LA   Luo Zhouqing Z   Fan Yanqun Y   Zhou Baojin B   Wen Bo B   Tan Fengji F   Wang Yujia Y   Zi Jin J   Xie Zexiong Z   Li Bingzhi B   Yang Kun K   Richardson Sarah M SM   Jiang Hui H   French Christopher E CE   Nieduszynski Conrad A CA   Koszul Romain R   Marston Adele L AL   Yuan Yingjin Y   Wang Jian J   Bader Joel S JS   Dai Junbiao J   Boeke Jef D JD   Xu Xun X   Cai Yizhi Y   Yang Huanming H  

Science (New York, N.Y.) 20170301 6329

Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII,  ...[more]

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