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Saccharomyces cerevisiae

ABSTRACT: Deep functional analysis of synII, a 770 kb synthetic yeast chromosome

OTHER RELATED OMICS DATASETS IN: MTBLS296PXD003435

PROVIDER: PRJNA352710 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5000700.fastq.gz	Fastqsanger.gz
	SRR5000697.fastq.gz	Fastqsanger.gz
	SRR5000698.fastq.gz	Fastqsanger.gz
	SRR5000699.fastq.gz	Fastqsanger.gz

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Deep functional analysis of synII, a 770 kb synthetic yeast chromosome

Project description:Here we report the design, construction and characterization of a 770 Kb synthetic yeast chromosome II (synII). With the UPLC-MS method, we demonstrated synII maintains a nearly wild-type phenotype and fitness despite hundreds of designer editing to it. Our phenomics, transcriptomics, proteomics data futher supports the identity of synII and wild one.

2016-10-13 | MTBLS296 | MetaboLights

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Deep functional analysis of synII, a 770 kb synthetic yeast chromosome

Project description:Herein we report the successful design, construction and characterization of a 770 kb synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels, including phenomics, transcriptomics, proteomics, chromosome segregation and replication analysis to provide the most thorough and comprehensive analysis of a synthetic chromosome. Our “Trans-Omics” analyses reveal a modest but potentially significant pervasive up-regulation of translational machinery observed in synII is mainly caused by the deletion of 13 tRNAs. By both complementation assays and SCRaMbLE, we targeted and debuged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the HOG response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.

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RNA sequence (RNA-seq) profiling of synthesized chromosome SynI in Saccharomyces cerevisiae

Project description:We designed and synthesized synI, which is ~21.4% shorter than native chrI, the smallest chromosome in Saccharomyces cerevisiae. SynI was designed for attachment to another synthetic chromosome due to concerns surrounding potential instability and karyotype imbalance, and is now attached to synIII, yielding the first synthetic yeast fusion chromosome. We constructed additional fusion chromosomes to investigate effects of fusions on nuclear function. We observed unexpected loops and twisted structures in chrIII-I and chrIX-III-I fusion chromosomes dependent on silencing protein Sir3. ChrI faces special challenges in assuring meiotic crossovers required for efficient homolog disjunction. Centromere deletions engineered into fusion chromosomes revealed opposing effects of core centromeres and pericentromeres in modulating deposition of meiotic recombination protein Red1. These effects extended over >100kb, to disproportionally promote meiotic recombination of small chromosomes like chrI. These findings reveal the power of synthetic genomics to uncover new biology and deconvolute complex biological systems.

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MetaboLights MTBLS296 - GNPS Deep functional analysis of synII, a 770 kb synthetic yeast chromosome

Project description:This data set was downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS296 Abstract:Here we report the design, construction and characterization of a 770 Kb synthetic yeast chromosome II (synII). With the UPLC-MS method, we demonstrated synII maintains a nearly wild-type phenotype and fitness despite hundreds of designer editing to it. Our phenomics, transcriptomics, proteomics data futher supports the identity of synII and wild one.

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