Proteomics

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Synthetic yeast genome project

ABSTRACT: The experiment aimed to study the proteome variation in a yeast strain (YS031), which chromosome II was artificially synthezied, compared with the wild type strain (BY4741). Proteins were extracted from the yeast cells and digested with trypsin. The peptides were label with iTRAQ, separated with 2D LC (SCX and RP) and detected with a Q-Exactive MS. Proteome identification and quantification were performed with Mascot2.3 and iQuant3.0.

OTHER RELATED OMICS DATASETS IN: MTBLS296PRJNA352710

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Baojin Zhou  

LAB HEAD: Bo Wen

PROVIDER: PXD003435 | Pride | 2018-10-27

REPOSITORIES: Pride

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Publications

Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome.

Shen Yue Y   Wang Yun Y   Chen Tai T   Gao Feng F   Gong Jianhui J   Abramczyk Dariusz D   Walker Roy R   Zhao Hongcui H   Chen Shihong S   Liu Wei W   Luo Yisha Y   Müller Carolin A CA   Paul-Dubois-Taine Adrien A   Alver Bonnie B   Stracquadanio Giovanni G   Mitchell Leslie A LA   Luo Zhouqing Z   Fan Yanqun Y   Zhou Baojin B   Wen Bo B   Tan Fengji F   Wang Yujia Y   Zi Jin J   Xie Zexiong Z   Li Bingzhi B   Yang Kun K   Richardson Sarah M SM   Jiang Hui H   French Christopher E CE   Nieduszynski Conrad A CA   Koszul Romain R   Marston Adele L AL   Yuan Yingjin Y   Wang Jian J   Bader Joel S JS   Dai Junbiao J   Boeke Jef D JD   Xu Xun X   Cai Yizhi Y   Yang Huanming H  

Science (New York, N.Y.) 20170301 6329

Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII,  ...[more]

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