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ProteomicsProteomics Using Proteases Alternative to Trypsin Benefits from Sequential Digestion with Trypsin
High specificity and ease of use make trypsin the most used enzyme in proteomics. Proteases with complementary cleavage specificity to trypsin have been applied to obtain additional data. However, use of proteases with broad specificity proved especially challenging. In this work, we analyzed the ch...
ORGANISM(S): Schizosaccharomyces pombe OY26 
2021-06-07 | PXD017321 | Pride
Alternative proteases Sequential digest 
ProteomicsSequential digestion with Trypsin and Elastase in cross-linking/mass spectrometry
Cross-linking/mass spectrometry has become an important approach for studying protein structures and protein-protein interactions. The amino acid composition of some protein regions impedes the detection of cross-linked residues, although it would yield invaluable information for protein modelling. ...
ORGANISM(S): Homo sapiens (Human) Schizosaccharomyces pombe 
2019-03-07 | PXD011459 | Pride
Lc-msms Cross-linking Yeast Human taf4-12 complex 
ProteomicsAlphaLink: bringing protein structure prediction in situ with in-cell photo-crosslinking mass spectrometry, crosslinking mass spectrometry, XL-MS,
The protein structure prediction problem has been revolutionised by AlphaFold2, an algorithm that uses neural networks and evolutionary information to predict accurate models from the primary sequence. However, some proteins remain difficult to predict. Moreover, proteins are dynamic entities that e...
ORGANISM(S): Escherichia Coli 
2023-02-22 | PXD036833 | JPOST Repository
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