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Michelle N. Arbeitman, Eileen E. M. Furlong, Farhad Imam, Eric Johnson, Brian H. Null, Bruce S. Baker, Mark A. Krasnow, Matthew P. Scott, Ronald W. Davis and Kevin P. White. Gene Expression During the Life Cycle of Drosophila melanogaster, Science, 297: 2270-2275, 2002. This work can be accessed fro...
ORGANISM(S): Drosophila melanogaster 
These experiments were conducted as part of a study to derive the targets of miRNAs. Two independent BayGenomics mouse embryonic stem cell lines (XH157 and XG058), each bearing a gene trap between exons 9 and 10 of the Dgcr8 locus (ENSMUST00000115633, Ensembl v53) were further mutagenised by the tar...
ORGANISM(S): Mus musculus 
To determine the role of Ascl1 in beta cell development, function, and metabolic stress response, we generated beta cell specific Ascl1 knockout mice and assessed their glucose homeostasis, islet morphology, and gene expression after feeding a normal diet, a high fat diet (HFD) for 12 weeks, or on a...
ORGANISM(S): Mus musculus 
To reprogram mouse embryonic fibroblasts (MEFs) to induced Pluripotent Stem Cells (iPSCs), we constructed the PiggyBac (PB) transposon carrying the four Yamanaka factor cDNAs controlled by a CAG promoter (PB-CAG-OCKS, Oct4, cMyc, Klf4 and Sox2). As the baseline reprogramming control, we transfected ...
ORGANISM(S): Mus musculus 
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