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Comparison of gene expression in cancer cell lines treated with rapamycin for 24h compared to DMSO
ORGANISM(S): Homo sapiens 
Investigation of differential pre-mRNA splicing in breast cancer cell line MCF-7 treated with rapamycin compared to vehicle DMSO that are overexpressing TRIB3
ORGANISM(S): Homo sapiens 
Previous reports have described the use of microarrays to assess the molecular classification of human breast cancers and defined new subgroups based on gene expression that are relevant to patient management through their ability to predict metastatic relapse and survival relapse. However, differen...
ORGANISM(S): Homo sapiens 
RNA was extracted with Rneasy Micro Kit (Qiagen, France) from SKBR3 cells treated with 1 uM ATRA. The quantity and purity of the extracted RNA was evaluated using a NanoDropTM spectrophotometer and its integrity measured using an Agilent BioanalyzerTM. For microarray hybridizations, 500 ng of total ...
ORGANISM(S): Homo sapiens 
Background. Although gene expression arrays have been used to generate molecular predictors of relapse and drug sensitivity in breast cancer, no large study describes genes and exons differentially expressed between breast cancer and benign lesions.

Methods. One hundred and sixty-five tumour...
ORGANISM(S): Homo sapiens 
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