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ProteomicsTDFragMapper: Evaluating experimental parameters for confident intact protein characterization in top-down proteomics
New software-tool allowing an easy visualization of fragment ions and thus a rapid evaluation of key experimental parameters on the sequence coverage obtained for the MS/MS analysis of intact proteins. Our tool can deal with multiple fragmentation methods. We demonstrate that TDFragMapper can rapidl...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Streptococcus dysgalactiae Escherichia coli 
2021-12-30 | PXD024643 | Pride
Visualization tool Top-down proteomics Lc-ms/ms 
ProteomicsA multi-parameter optimization in middle-down analysis of monoclonal antibodies by LC-MS/MS
In antibody-based drug research, regulatory agencies request a complete characterization of antibody proteoforms covering both the amino acid sequence and all post-translational modifications. The usual mass spectrometry-based approach to achieve this goal is bottom-up proteomics, which relies on th...
ORGANISM(S): Homo sapiens (Human) 
2023-03-15 | PXD038619 | Pride
Orbitrap ms Middle-down proteomics Monoclonal antibody Intact proteins Lc-ms/ms 
ProteomicsMonitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry
We propose here a workflow based on an automated immunocapture followed by top-down and middle-down LC-MS/MS approaches to characterize mAb proteoforms spiked in mouse plasma. We demonstrate the applicability of our workflow on a large concentration range using pembrolizumab as a model. We also comp...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-08-27 | PXD040896 | Pride
Immunocapture Monoclonal antibody Proteoforms Middle-down mass spectrometry Top-down mass spectrometry 
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