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TranscriptomicsIn vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs
Dicer is an RNase III-family endoribonuclease and haploinsufficient tumor suppressor that is required for the biogenesis of miRNAs, yet in vivo structure-function characterization of its RNase IIIA and IIIB domains have not been reported. In murine Dicer knockout fibroblasts, we expressed human Dic...
ORGANISM(S): Mus musculus 
2012-07-13 | E-GEOD-36978 | biostudies-arrayexpress
TranscriptomicsEndogenous shRNAs, siRNAs, and Other Microprocessor-independent, Dicer-dependent Small RNAs in Mouse ES Cells
Canonical microRNAs (miRNAs) require two processing steps: the first by the Microprocessor, a complex of DGCR8 and Drosha, and the second by Dicer. dgcr8delta/delta mouse embryonic stem cells (mESCs) have less severe phenotypes than dicer1delta/delta mESCs, suggesting a physiological role for Microp...
ORGANISM(S): Mus musculus 
2008-09-09 | E-GEOD-12521 | biostudies-arrayexpress
TranscriptomicsDicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [RT-PCR]
MicroRNAs (miRNAs) are small, endogenous, non-protein coding RNAs that are an important means of post-transcriptional gene regulation. Deletion of Dicer, a key miRNA processing enzyme, is embryonic lethal in mice, and tissue-specific Dicer deletion results in developmental defects. Using a condition...
ORGANISM(S): Mus musculus 
2013-05-01 | E-GEOD-45810 | biostudies-arrayexpress
TranscriptomicsTranscriptome wide identification of Dicer binding in human and C. elegans reveals a variety of substrates (CEL RNA-Seq)
Dicer is a deeply conserved endoribonuclease with key functions in small RNA biogenesis. Here we employed PAR-CLIP/iPAR-CLIP to identify direct Dicer binding sites in the transcriptomes of human cells and C.elegans. We found hundreds of novel miRNAs and non-canonical Dicer substrates with high sensi...
ORGANISM(S): Caenorhabditis elegans 
2014-11-20 | E-GEOD-55329 | biostudies-arrayexpress
TranscriptomicsThe effects of the 5' pocket motif of Dicer on miRNA biogenesis
A hallmark of RNA silencing is a class of ~22 nt RNAs which are processed from dsRNA precursor by Dicer. Accurate processing by Dicer is critical for the functionality of microRNAs (miRNAs). According to the current model, Dicer measures the length by anchoring the 3' overhang of the dsRNA terminus....
ORGANISM(S): Mus musculus 
2011-07-12 | E-GEOD-27903 | biostudies-arrayexpress
TranscriptomicsDeletion of astroglial Dicer causes cell non-autonomous neuronal dysfunction and degeneration
The endoribonuclease, Dicer, is indispensible for generating the majority of mature microRNAs (miRNAs), which are posttranscriptional regulators of gene expression involved in a wide range of developmental and pathological processes in mammalian central nervous system. While functions of Dicer-depen...
ORGANISM(S): Mus musculus 
2011-05-10 | E-GEOD-28589 | biostudies-arrayexpress
TranscriptomicsA variety of Dicer substrates in human and C. elegans (CEL RNA-seq)
The endoribonuclease Dicer is known for its central role in the biogenesis of eukaryotic small RNAs/microRNAs. Despite its importance, Dicer target transcripts have not been directly mapped. Here, we apply biochemical methods to human cells and C. elegans and identify thousands of Dicer binding site...
ORGANISM(S): Caenorhabditis elegans 
2014-11-20 | E-GEOD-63435 | biostudies-arrayexpress
ProteomicsDICER-interacting partners in juvenile mouse testes
DICER has a well-characterized role in the processing of microRNAs (miRNAs) and small interfering RNAs (siRNA) that are important for post-transcriptional gene regulation. Emerging evidence suggests that DICER also has several non-canonical functions beyond miRNA/siRNA biogenesis, for example in tra...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-03 | PXD017772 | Pride
Mouse testis Germ cells Dicer 
TranscriptomicsTranscriptome wide identification of Dicer binding in human and C. elegans reveals a variety of substrates (HEK RNA-Seq)
Dicer is a deeply conserved endoribonuclease with key functions in small RNA biogenesis. Here we employed PAR-CLIP/iPAR-CLIP to identify direct Dicer binding sites in the transcriptomes of human cells and human. We found hundreds of novel miRNAs and non-canonical Dicer substrates with high sensitivi...
ORGANISM(S): Homo sapiens 
2014-11-20 | E-GEOD-55326 | biostudies-arrayexpress
TranscriptomicsHuman nuclear Dicer restricts the deleterious accumulation of endogenous double strand RNA
Dicer is a central enzymatic player in RNA interference (RNAi) pathways that acts to regulate gene expression in nearly all eukaryotes. Although the cytoplasmic function of Dicer is well documented in mammals, there is little known about any possible nuclear function. Here we show that Dicer is pre...
ORGANISM(S): Homo sapiens 
2014-04-10 | E-GEOD-56648 | biostudies-arrayexpress
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