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TranscriptomicsPax5 restoration in a mouse model of B progenitor acute lymphoblastic leukemia
Hypomorphic mutations of PAX5 occur in one third of B-progenitor acute lymphoblastic leukemias (B-ALLs), however their functional consequences remain undefined. Here we employ advanced transgenic RNAi in mice to suppress endogenous Pax5 expression in the hematopoietic compartment in vivo, which co-o...
ORGANISM(S): Mus musculus 
2014-06-18 | E-GEOD-52870 | biostudies-arrayexpress
TranscriptomicsModeling tumor subtypes in vivo using lineage restricted transgenic shRNA
Expression analysis from two genetically engineered mouse models of osteosarcoma determine the expression profile of mouse osteosarcoma Human osteosarcoma (OS) is comprised of three different subtypes: fibroblastic, chondroblastic and osteoblastic. We previously generated a mouse model of fibroblast...
ORGANISM(S): Mus musculus 
2013-03-28 | E-GEOD-38742 | biostudies-arrayexpress
TranscriptomicsExpression profiling of mouse T-ALL (Vav-tTA;TRE-GFP-shIkaros primary leukemia ALL101) cells following Ikaros restoration [RNA-Seq]
To examine Ikaros tumor suppressor mechanisms, we have utilized inducible RNAi to dynamically restore endogenous Ikaros expression in T-ALL driven by its knockdown. This causes rapid transcriptional repression of Notch1 and associated targets including Myc, even in leukemias harboring spontaneous ac...
ORGANISM(S): Mus musculus 
2015-02-02 | E-GEOD-54447 | biostudies-arrayexpress
TranscriptomicsExpression profiling of mouse bone marrow pre-B cells
Triplicate RNA-seq expression analysis of bone marrow pre-B cells isolated from mice, to demonstrate repertoire at the IgH locus Triplicate RNA-seq expression analysis of bone marrow pre-B cells
ORGANISM(S): Mus musculus 
2014-06-18 | E-GEOD-52868 | biostudies-arrayexpress
TranscriptomicsStage-specific control of early B cell development by the transcription factor Ikaros
Ikaros is an essential regulator of lymphopoiesis. Here, we studied the B-cell-specific function of Ikaros by conditional Ikzf1 inactivation in pro-B cells. B-cell development was arrested at an aberrant ‘pro-B’ cell stage characterized by increased cell adhesion and loss of pre-B cell receptor sign...
ORGANISM(S): Mus musculus 
2014-02-09 | E-GEOD-53595 | biostudies-arrayexpress
TranscriptomicsInducible PAX5 expression in a human B lymphoblastic leukemia cell line
Hypomorphic mutations of the transcription factor PAX5 occur in one third of B-progenitor acute lymphoblastic leukemias (B-ALLs). To identify PAX5-regulated genes in B-ALL, here we employ inducible expression of PAX5 in a human B-ALL cell line (REH) that harbors a loss-of-function mutation in PAX5. ...
ORGANISM(S): Homo sapiens 
2014-06-18 | E-GEOD-57480 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human IMR90 cells after RNAi suppression of RB, p107 or p130 using in growing, quiescent or ras-induced senescent IMR90 cells
The action of RB as a tumor suppressor has been difficult to define, in part, due to the redundancy of the related proteins p107 and p130. By coupling advanced RNAi technology to suppress RB, p107 or p130 with a genome wide analysis of gene expression in growing, quiescent or ras-senescent cells, we...
ORGANISM(S): Homo sapiens 
2010-01-23 | E-GEOD-19864 | biostudies-arrayexpress
TranscriptomicsA rapid and scalable system for studying gene function in mice using conditional RNA interference
Four libraries were analyzed to assess the level and effects of inducing the ectopic expression of a luciferase targeting miRNA in mouse embryonic fibroblasts (MEFs). Here, we find low level, leaky expression in the absence of ROSA-rtTA and doxycyclin. When expression is effectively induced, the Luc...
ORGANISM(S): Mus musculus 
2010-12-01 | E-GEOD-19476 | biostudies-arrayexpress
UnknownARF functions as a melanoma tumor suppressor by inducing p53-independent senescence.
Not available
S-EPMC1904138 | biostudies-literature
UnknownCombined targeting of JAK2 and Bcl-2/Bcl-xL to cure mutant JAK2-driven malignancies and overcome acquired resistance to JAK2 inhibitors.
Not available
S-EPMC3898474 | biostudies-literature
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