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TranscriptomicsNANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers
Nanog, a core pluripotency factor in the inner cell mass of blastocysts, is also expressed in unipotent primordial germ cells (PGC) in mice1, where its precise role is yet unclear2-4. We investigated this in an in vitro model, where naïve pluripotent embryonic stem cells (ESCs) cultured in bFGF/Act...
ORGANISM(S): Mus musculus 
2016-01-11 | E-GEOD-71933 | biostudies-arrayexpress
TranscriptomicsSOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate
Specification of primordial germ cells (PGCs) marks the beginning of the totipotent state. However, without a tractable experimental model, the mechanism of human PGC (hPGC) specification remains unclear. Here, we demonstrate specification of hPGC-like cells (hPGCLCs) from germline competent pluripo...
ORGANISM(S): Homo sapiens 
2014-12-26 | E-GEOD-60138 | biostudies-arrayexpress
TranscriptomicsStem cell function and sensitivity to genotoxic stress is controlled by protein translation rates
Whether protein synthesis and cellular stress response pathways interact to control stem cell functions is currently unknown. Here, we show that skin stem cells synthesise less protein than their immediate progenitors in vivo, even when forced to proliferate in a tumour model. Our analyses reveal th...
ORGANISM(S): Mus musculus 
2016-06-16 | E-GEOD-72067 | biostudies-arrayexpress
TranscriptomicsRibosome Profiling of mouse skin squamous tumours
Purpose: Ribosome profiling has revolutionized systems-based analysis and which produces a “global snapshot” of all the ribosomes translationally active in a cell at a particular moment. The goals of this study are to first apply ribosome profiling to in vivo samples for the first time and in pa...
ORGANISM(S): Mus musculus 
2016-06-16 | E-GEOD-72066 | biostudies-arrayexpress
GenomicsNANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers [ChIP-seq]
Nanog, a core pluripotency factor in the inner cell mass of blastocysts, is also expressed in unipotent primordial germ cells (PGC) in mice1, where its precise role is yet unclear2-4. We investigated this in an in vitro model, where naïve pluripotent embryonic stem cells (ESCs) cultured in bFGF/Act...
ORGANISM(S): Mus musculus 
2016-01-11 | E-GEOD-71932 | biostudies-arrayexpress
TranscriptomicsPRMT5 protects genomic integrity during global DNA demethylation in primordial germ cells and preimplantation embryos
Primordial germ cells (PGCs) and preimplantation embryos undergo epigenetic reprogramming, which entails comprehensive erasure of DNA methylation. We found that PRMT5, an arginine methyltransferase, translocates from the cytoplasm to the nucleus during this process. Here we show that conditional los...
ORGANISM(S): Mus musculus 
2014-11-14 | E-GEOD-60875 | biostudies-arrayexpress
GenomicsTransfer RNA (tRNA) processing and RNA m5C-methylation profiles of mouse skin squamous tumours
Purpose: High-throughput RNA sequencing has accelerated discovery of the complex regulatory roles of small RNAs, such those derived from tRNAs. Also recent advances in high-throughput RNA sequencing has revealed the complex RNA modification landscape and the complex role these nucleosides modifactio...
ORGANISM(S): Mus musculus 
2016-06-16 | E-GEOD-72065 | biostudies-arrayexpress
TranscriptomicsNANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers [microarray]
Nanog, a core pluripotency factor in the inner cell mass of blastocysts, is also expressed in unipotent primordial germ cells (PGC) in mice1, where its precise role is yet unclear2-4. We investigated this in an in vitro model, where naïve pluripotent embryonic stem cells (ESCs) cultured in bFGF/Act...
ORGANISM(S): Mus musculus 
2016-01-11 | E-GEOD-61924 | biostudies-arrayexpress
TranscriptomicsRNA-seq of chemically reset human naive pluripotent stem cells
A simple method is presented to reset human pluripotent cells to a naive state via transient histone deacetylase inhibition and maintenance in chemically-defined naive stem cell culture media. Cells can be reset without transgenes and expanded continuously either on feeders or alternative substrates...
ORGANISM(S): Homo sapiens 
2017-08-07 | E-MTAB-5674 | biostudies-arrayexpress
GenomicsChromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
Early mouse development is accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2), which is essential for embryogenesis. Here we show that H3K9me2 directs repression of 2-cell stage specific genes in nascent embryos to facilitate...
ORGANISM(S): Mus musculus 
2015-11-09 | E-GEOD-70355 | biostudies-arrayexpress
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