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GenomicsDnmt2-dependent methylomes lack biologically relevant DNA methylation patterns
Several organisms belonging to diverse animal groups have retained Dnmt2 as their only bona fide DNA methyltransferase gene. However, recent studies have shown that Dnmt2 functions as a tRNA methyltransferase, which prompted us to analyze the methylomes of Dnmt2-only organisms at single-base resolut...
ORGANISM(S): Mus musculus 
2013-05-06 | E-GEOD-42170 | biostudies-arrayexpress
GenomicsDnmt2-dependent methylomes lack biologically relevant DNA methylation patterns [Drosophila melanogaster]
Dnmt2 is a widely conserved protein, which is closely related to eukaryotic DNA methyltransferases. However, Dnmt2 shows a robust tRNA methyltransferase activity and only limited activity towards DNA. Interestingly, a recent study has provided evidence for a biologically important function of Dnmt2-...
ORGANISM(S): Drosophila melanogaster 
2013-05-06 | E-GEOD-39996 | biostudies-arrayexpress
GenomicsHigh resolution transcriptome-wide RNA cytosine methylome of Mouse Embryonic Fibroblasts
We report high resolution transcriptome-wide RNA cytosine methylome of Mouse Embryonic Fibroblasts (MEFs) revealed by an optimized new RNA bisulfite sequencing approach. Comparison of RNA methylation profile from wild-type and Dnmt2 -/- MEFs shows that only C38 in three tRNAs (tRNA-Asp, Gly and Val...
ORGANISM(S): Mus musculus 
2013-04-21 | E-GEOD-44359 | biostudies-arrayexpress
GenomicsDnmt2-dependent methylomes lack biologically relevant DNA methylation patterns [Schistosoma mansoni]
Dnmt2 is a widely conserved protein, which is closely related to eukaryotic DNA methyltransferases. However, Dnmt2 shows a robust tRNA methyltransferase activity and only limited activity towards DNA. Interestingly, a recent study has provided evidence for a biologically important function of Dnmt2-...
ORGANISM(S): Schistosoma mansoni 
2013-05-06 | E-GEOD-39997 | biostudies-arrayexpress
TranscriptomicsHigh-throughput sequencing of small RNAs (25-70 nt) from Drosophila wild-type and Dnmt2 mutants
Dnmt2 genes are highly conserved tRNA methyltransferases with biological roles in cellular stress responses. The absence of obvious mutant phenotypes under laboratory conditions suggested a function for Dnmt2 under non-physiological conditions. Indeed, Dnmt2 has recently been implicated in various a...
ORGANISM(S): Drosophila melanogaster 
2012-11-12 | E-GEOD-35981 | biostudies-arrayexpress
ProteomicsIdentification of Protein associations of Human DNMT2/TRDMT1 during stress response
Data-dependent acquisition tandem mass spectrometry was performed to characterize the proteins co-immunoprecipitated with the ectopically expressed DNMT2/TRDMT1-FLAG-HA. Protein extracts were obtaind from Dox-induced Flp-In™ T-REx™ 293 cells that were kept at steady-state or during the response to t...
ORGANISM(S): Homo sapiens (Human) 
2026-10-05 | PXD083610 | Pride
Dnmt2 Co-immunoprecipitation Trdmt1 Cellular stress 
TranscriptomicsSite-specific cytosine-5 methylation by the mouse RNA methyltransferases Dnmt2 and NSun2 promotes tRNA stability and protein synthesis
Mouse embryonic fibroblast (MEFs) cell lines and liver samples from Dnmt2 and Nsun2 single mutant, double mutant and wildtype mice were used to identify potential mRNA substrates of both proteins. MEFs were derived from day 13.5 embryos and immortalized by transfection with a plasmid expressing the ...
ORGANISM(S): Mus musculus 
2012-07-03 | E-GEOD-36918 | biostudies-arrayexpress
TranscriptomicsAberrant haematopoiesis in Dnmt2 mutant mice [array]
Bone marrow samples from Dnmt2-/- and wildtype mice were used to identify potential mRNA substrates of Dnmt2
ORGANISM(S): Mus musculus 
2015-08-13 | GSE65302 | GEO
Methylation profilingDnmt2-dependent methylomes lack biologically relevant DNA methylation patterns
Several organisms belonging to diverse animal groups have retained Dnmt2 as their only bona fide DNA methyltransferase gene. However, recent studies have shown that Dnmt2 functions as a tRNA methyltransferase, which prompted us to analyze the methylomes of Dnmt2-only organisms at single-base resolut...
ORGANISM(S): Mus musculus 
2013-05-06 | GSE42170 | GEO
UnknownOn the evolutionary origin of eukaryotic DNA methyltransferases and Dnmt2.
Not available
S-EPMC3227630 | biostudies-literature
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