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TranscriptomicsZebrafish (Danio rerio) catecholaminergic neuron RNA-seq based transcript profiles
This project aimed at identifying developmental stage specific transcript profiles for catecholaminergic neurons in embryos and early larvae of zebrafish (Danio rerio). Catecholaminergic neurons were labeled using transgenic zebrafish strains to drive expression of GFP. At stages 24, 36, 72 and 96 h...
ORGANISM(S): Danio rerio 
2012-10-15 | E-GEOD-41373 | biostudies-arrayexpress
TranscriptomicsExperiment 4: Affymetrix validation array
Zebrafish embryo were analyzed at 30 and 60 % epiboly for changes in transcriptome of wild-type and MTspg mutant embryos The Data are used as an independent experimental validation of data obtained for the same stages by Agilent arrays in the main experiments (especially Exp. 1) Experiments were per...
ORGANISM(S): Danio rerio 
2010-03-07 | E-GEOD-17657 | biostudies-arrayexpress
TranscriptomicsExperiment 2: Pou5f1 and Sox2 overexpression experiments protein synthesis inhibition
Pou5f1 and Sox2 overexpression experiments with protein synthesis inhibitor were performed to investigate direct transcriptional targets of Pou5f1 and Sox2 Experiments were performed in biological triplicates. Cy3 and Cy5 channels were split and analyzed separately using Genedata Analyst software, q...
ORGANISM(S): Danio rerio 
2013-07-31 | E-GEOD-41107 | biostudies-arrayexpress
TranscriptomicsExperiment 3: Klf2a and Klf2b loss-of-function experiments at 30% epiboly Wild Type zebrafish embryos
Morpholino injections were performed to investigate transcriptional targets of Klf2a, and Klf2b in the blastula stage embryos (4,7 hours postfertilization) of wild type zebrafish embryos. Experiments were performed in biological duplicates. Cy3 and Cy5 channels were splitted in Genedata Analyst sof...
ORGANISM(S): Danio rerio 
2013-12-31 | E-GEOD-45011 | biostudies-arrayexpress
TranscriptomicsExperiment 4: Klf4, FoxD3, FoxD5 and combined FoxD3 and FoxD5 loss-of-function experiments at 30% epiboly Wild Type zebrafish embryos
Morpholino injections were performed to investigate transcriptional targets of Klf4, FoxD3 and FoxD5 in the blastula stage embryos (4,7 hours postfertilization) of wild type zebrafish embryos. Experiments were performed in biological duplicates or triplicates. Cy3 and Cy5 channels were splitted in ...
ORGANISM(S): Danio rerio 
2013-12-31 | E-GEOD-45012 | biostudies-arrayexpress
TranscriptomicsExperiment 2: Klf2a, Klf2b and FoxD3 overexpression experiments at 30% epiboly MZspg and WT embryos with or without protein synthesis inhibition
Overexpression experiments with or without protein synthesis inhibition were performed to investigate transcriptional targets of Klf2a, Klf2b and FoxD3 in the blastula stage embryos (4,7 hours postfertilization) of wild type or MZspg backgroumd Experiments were performed in biological duplicates. Cy...
ORGANISM(S): Danio rerio 
2013-12-31 | E-GEOD-45010 | biostudies-arrayexpress
TranscriptomicsTranscriptional targets of Pou5f1 in Zebrafish (Danio Rerio)
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Danio rerio 
2013-12-31 | E-GEOD-45013 | biostudies-arrayexpress
GenomicsZygotic gene activatory network in zebrafish is globally controlled by Pou5f1 SoxB1 enhancers
Genetic control of pluripotent mammalian ES cells is determined by a transcriptional network, with a "central core" of transcription factors, Pou5f1, Sox2 and Nanog. Zebrafish homologues of the "core pluripotency factors" Pou5f1, SoxB1 and Nanog-like are also crucially involved i...
ORGANISM(S): Danio rerio 
2013-08-10 | E-GEOD-39780 | biostudies-arrayexpress
TranscriptomicsExperiment 1: Overexpression experiments at 30% epiboly MZspg and WT embryos with or without protein synthesis inhibition
Overexpression experiments with or without protein synthesis inhibition were performed to investigate transcriptional targets of Klf4, FoxD3, and Nanog-like in the blastula stage embryos (4.7 hours postfertilization) of wild type or MZspg background. Experiments were performed in biological triplica...
ORGANISM(S): Danio rerio 
2013-12-31 | E-GEOD-44982 | biostudies-arrayexpress
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