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In the present paper, we introduce dithiothreitol (DTT) as a potent protein-RNA cross-linker. To prove this three model systems, a) a small synthetic peptide from smB'/B protein incubated with U1snRNA oligonucleotide, b) in vitro reconstituted 15.5K protein with U4 snRNA oligonucleotide and c) nativ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2015-10-13 | PXD002656 | Pride
Effect of Thiol reductive stress (DTT 1mM) on M.tb Rv was studied. DTT reduced at 1 mM concentration was added to log pahse culture of M.tb H37Rv. After Three hours RNA was isolated and hybridization was done on HRI-UMNDJ Mycobacterium tuberculosis 4.8K CAG_Mtb microarrays.
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
Transcriptome responsiveness was further tested by attempts to invoke the unfolded protein repsonse (UPR), a classic ER-based pathway stimulated by the presence of increased levels of unfolded polypeptides. The UPR is mediated via transcriptional responses in both yeast and metazoan cells, and can b...
ORGANISM(S): Trypanosoma brucei 
In the present paper, we introduce dithiothreitol (DTT) as a potent protein-RNA cross-linker. To prove this three model systems, a) a small synthetic peptide from smB'/B protein incubated with U1snRNA oligonucleotide, b) in vitro reconstituted 15.5K protein with U4 snRNA oligonucleotide and c) nativ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2022-03-01 | PXD002392 | Pride
Schizosaccharomyces pombe DTT treatment
Effect of Thiol reductive stress (DTT 5mM) on M.tb Rv was studied.
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2016-04-14 | GSE77848 | GEO
Effect of Thiol reductive stress (DTT 1mM) on M.tb Rv was studied.
ORGANISM(S): Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv 
2015-04-28 | GSE68350 | GEO
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