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Yeast cells were incubated at 25˚C, unless otherwise stated. For SILAC, cells were grown in light labeled (0.03mg/ml Lys0, 0.02mg/ml Arg0) or heavy labeled (0.03mg/ml Lys4, 0.02mg/ml Arg6) amino acid in synthetic defined medium with 2% dextrose for at least 7 generations. heat shock was performed i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-06-12 | PXD012631 | Pride
A central remaining question in the post-genomic era is how genes interact to form biological pathways. Measurements of gene dependency across hundreds of cell lines have been used to cluster genes into ‘co-essential’ pathways, but this approach has been limited by ubiquitous false positives. Here, ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-16 | PXD023558 | Pride
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