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T cells were extracted from spleens of Cas9 mice and activated in vitro with CD3/28 on day 0. They were spinoculated with retroviruses containing sgRNA on day 1. On day 2 and forwarded they were treated with puromycin. On day 4, dead cells were removed by low-G spin and the remaining cells measured ...
ORGANISM(S): Mus musculus 
CD4+ T cells were extracted from mouse and human. They were activated in vitro with CD3/28 and cultured with Il4. ChIPmentation was then performed
ORGANISM(S): Mus musculus 
CD4+ T cells were extracted from mouse and human. They were activated in vitro with CD3/28 and cultured with Il4. ATAC-seq was then performed at different time points
ORGANISM(S): Homo sapiens 
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