Sort   by:  
 Page size 
Transcriptomics3’RNA-seq of murine fresh and expanded ASPCs
To characterize murine ASPCs we performed bulk RNA-seq (BRB-seq, Alpern et al., 2019) of total, CD142− and CD142+ (Aregs) mouse adipose stem and progenitor cells (ASPCs). ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ cells of the mouse subcutaneous stromal vascular fraction us...
ORGANISM(S): Mus musculus 
2022-07-04 | E-MTAB-10179 | biostudies-arrayexpress
Transcriptomics3’RNA-seq of murine CD142− and CD142+ ASPCs freshly sorted with four different anti-CD142 antibodies
To validate that the robustness of Aregs' (CD142+ ASPCs') molecular identity regardless of the antibody used, we performed transcriptomic profiling of freshly isolated CD142− and CD142+ mouse adipose stem and progenitor cells (ASPCs) sorted using four different anti-CD142 antibodies. ASPCs were coll...
ORGANISM(S): Mus musculus 
2022-07-04 | E-MTAB-10181 | biostudies-arrayexpress
Transcriptomics3’RNA-seq of murine ASPCs derived from P0, P16, 4wo, 7wo and 11wo-mice
To explore the molecular identity of mouse adipose stem and progenitor cells (ASPCs) and more specifically of CD142+ ASPCs (Aregs), we performed bulk RNA-seq on freshly isolated total, CD142− and CD142+ ASPCs of new-born pups (P0), post-natal day 16 mice (P16), 4 week-old (4wo), 7wo and 11wo mice. A...
ORGANISM(S): Mus musculus 
2022-07-04 | E-MTAB-10182 | biostudies-arrayexpress
Transcriptomics3’RNA-seq of post-differentiation murine CD142− and CD142+ ASPCs sorted with four different anti-CD142 antibodies
To characterize CD142+ ASPCs (Aregs) after exposure to an adipogenic cocktail we performed bulk RNA-seq (using BRB-seq) of total, CD142− and CD142+ mouse adipose stem and progenitor cells (ASPCs), sorted using four different anti-CD142 antibodies. ASPCs were collected as Lin− (CD31− CD45− TER119−) C...
ORGANISM(S): Mus musculus 
2022-07-04 | E-MTAB-10180 | biostudies-arrayexpress
Transcriptomics3’RNA-seq of murine CD142− ASPCs co-cultured with CD142+ ASPCs (Aregs) carrying specific gene knockdowns
To study the involvement of key Areg (CD142+ ASPC)-specifics factors in the inhibitory capacity of CD142+ ASPCs on adipogenesis, we performed transcriptomic profiling of CD142− ASPCs exposed to the secretome of CD142+ ASPCs carrying knockdowns of the indicated genes. CD142− ASPCs were co-cultured ...
ORGANISM(S): Mus musculus 
2022-07-04 | E-MTAB-10183 | biostudies-arrayexpress
Transcriptomics3’RNA-seq of murine post-differentiation CD142− ASPCs treated with retinoic acid (RA)
To study the impact of retinoic acid treatment on the transcriptome of CD142− ASPCs, we performed bulk RNA-seq on CD142− ASPCs treated with different RA concentrations ranging between 0.01 to 100 μM, with 100 ng/ml of BSA (negative control), DMSO (negative control) or 100 ng/ml of EGF (positive cont...
ORGANISM(S): Mus musculus 
2021-06-26 | E-MTAB-10184 | biostudies-arrayexpress
Transcriptomics3’ RNA-seq of expanded undifferentiated and differentiated human ASPCs
Compare the transcriptomic signature of hASPCs isolated by plating the stromal vascular fraction of collagenase-digested human adipose tissue from four different depots at the undifferentiated (t0) and differentiated (t14) times.
ORGANISM(S): Homo sapiens 
2023-05-08 | E-MTAB-12898 | biostudies-arrayexpress
Sort   by:  
 Page size