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ProteomicsQuantification of mitochondrial translation by pulse SILAC
We developed a simple method that combined biochemical mitochondria isolation and pulse SILAC approach to monitor the mitochondrial translation. Our approach allows us to quantify 12 out of the 13 mitochondrial translation products, the highest coverage among analogous methods reported, and provide ...
ORGANISM(S): Homo Sapiens (human) 
2022-12-28 | PXD022476 | JPOST Repository
ProteomicsCystinosin turnover - Impact of cystinosin glycosylation on protein stability by differential dynamic SILAC
Cystinosis is a rare autosomal recessive lysosomal storage disorder, characterized by an intra-Cystinosis is a rare autosomal recessive lysosomal storage disorder, characterized by an intra-lysosomal accumulation of cystine. The causative gene for cystinosis is CTNS, which encodes the protein cystin...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2017-01-16 | PXD005357 | Pride
Cystinosis Er retention Dynamic silac Turnover Cystinosin 
UnknownSILACtor: software to enable dynamic SILAC studies.
Not available
S-EPMC3255570 | biostudies-literature
ProteomicsProtein turnover analysis in Salmonella Typhimurium during infection by dynamic SILAC, Topograph and quantitative proteomics
Protein turnover affects protein abundance and phenotypes. Comprehensive investigation of protein turnover dynamics has the potential to provide substantial information about gene expression. Here we report a large-scale protein turnover study in Salmonella Typhimurium during infection by quantitati...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium 
2022-05-17 | PXD033623 | Pride
Proteomics Salmonella typhimurium Dynamic silac Protein turnover 
ProteomicsHigh Dynamic Range peptide mass spectrometry using segmented precursor ions accumulation - SILAC
Limited sensitivity and depth of proteome sampling in experiments using Data-Dependent Acquisition (DDA) mass spectrometry are usually attributed to insufficient rate of fragmentation spectra acquisition relative to the number of co-eluting potential targets. Here we demonstrated that limited sensit...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000099210 | MassIVE
ProteomicsDynamic profiling of the protein life cycle in response to pathogens (protein)
Protein expression is regulated by production and degradation of mRNAs and proteins, but their specific relationships remain unknown. We combine measurements of protein production and degradation and mRNA dynamics to build a quantitative genomic model of the differential regulation of gene expressio...
ORGANISM(S): Mus musculus 
2015-03-06 | E-GEOD-59792 | biostudies-arrayexpress
ProteomicsPulse SILAC analysis of immuno-precipitated mitochondrial complexes I, IV and V
Pulse SILAC analysis of mitochondrial complexes I, IV and V HeLa cells
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-03 | MSV000084748 | MassIVE
ProteomicsPulse SILAC analysis of mitochondrial complex assembly
Pulse SILAC analysis of mitochondrial protein complex assembly Pulse times (hours): P3, P4, P6, P12 Bogenhagen et al. Cell Reports 2018
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-03 | MSV000084749 | MassIVE
ProteomicsDevelopment and evaluation of a SILAC method for proteomic analysis of rat primary microglia
Microglia play important and dynamic roles in mediating a variety of physiological and pathological processes during the development, normal function, and degeneration of the central nervous system. We report here the development of a SILAC-labeled model system suitable for mass spectrometry-based ...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-04-07 | PXD002759 | Pride
Rat Silac Microglia Primary 
ProteomicsSpatial SILAC Regorafenib Treated Bottom-up Proteomic Analysis
Developing effective model systems to evaluate new potential chemotherapeutic reagents is critical. Three-dimensional cell cultures, such as spheroids, aid in bridging the gap between commonly used monolayer cell cultures and significantly more complex and expensive animal models. Spheroid model sys...
ORGANISM(S): Homo sapiens (Human) 
2024-08-09 | PXD038958 | Pride
Spatial silac Regorafenib Spheroids 
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