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TranscriptomicsE coli MG1655 biofilm and planktonic gene expression
Bacteria growing as surface-adherent biofilms are better able to withstand chemical and physical stresses than their unattached, planktonic counterparts. Using transcriptional profiling and quantitative PCR, we observed a previously uncharacterized gene, yjfO, to be upregulated during Escherichia co...
ORGANISM(S): Escherichia coli 
2010-06-21 | E-GEOD-18362 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of E. coli MG1655
Expression profiling of wild type and purR deletion strains of E. coli K-12 MG1655 under both M9 minimal media and addition of adenine. An eight chip study with two different strains under two separate culture conditions.
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2011-06-14 | E-GEOD-26588 | biostudies-arrayexpress
TranscriptomicsCarbon nutrition of pathogenic and commensal E. coli
Transcriptome profiling of E. coli MG1655, E. coli EDL933, E. coli ECOR26, and E. coli Nissle 1917 grown on mouse cecal mucus as carbon source The carbon sources that support growth of pathogenic E. coli O157:H7 in the mammalian intestine have not previously been investigated. In vivo, pathogenic E...
ORGANISM(S): Escherichia coli 
2008-04-25 | E-GEOD-11254 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of a slyA mutant and a slyA-overexpression strain of Escherichia coli MG1655 in order to elucidate the SlyA regulon
A microarray study was performed to identify the Escherichia coli SlyA regulon. The wild-type (parental) Escherichia coli MG1655 strain was compared to an isogenic slyA deletion mutant, whilst a slyA-overexpression strain (in pET28a) was compared to an empty (pET28a) vector control. Continuous chemo...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2017-01-25 | E-MTAB-5220 | biostudies-arrayexpress
TranscriptomicsTime series analysis of glucose-lactose diauxie: involvement of stringent response
Guanosine 3 ,5 -bispyrophosphate (ppGpp), also known as ‘‘magic spot,’’ has been shown to bind prokaryotic RNA polymerase to down-regulate ribosome production and increase transcription of amino acid biosynthesis genes during the stringent response to amino acid starvation. Because many environmenta...
ORGANISM(S): Escherichia coli 
2007-03-17 | E-GEOD-7265 | biostudies-arrayexpress
TranscriptomicsMG1655 araC arabinose transcription profiling
AraC is an Escherichia coli transcription factor that regulates genes in response to the presence/absence of arabinose. We used transcription profiling to determine RNA levels in Escherichia coli K-12 strain MG1655 and MG1655 delta araC grown either in the absence or the presence of arabinose. Thus,...
ORGANISM(S): Escherichia coli K-12 
2013-10-19 | E-MTAB-1916 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli high-cell-density recombinant and wild-type
The transcriptome profiles for wild-type (plasmid-free) and recombinant (plasmid-bearing) Escherichia coli during well-controlled synchronized high-cell-density fed-batch cultures were analyzed by DNA microarrays. It was observed that the growth phase significantly affected the transcriptome profile...
ORGANISM(S): Escherichia coli 
2009-08-25 | E-GEOD-17505 | biostudies-arrayexpress
TranscriptomicsMixed Culture Gene Expression of E. coli and Pseudomonas aeruginosa grown on defined media with N-acetyl glucosamine
Transcriptional profiles of Escherichia coli MG1655 in mixed culture with Pseudomonas aeruginosa PAO1 showed a number of E. coli genes to be upregulated including purA-F and other genes associated with purine synthesis. In contrast, genes associated with pyrimidine synthesis were unaffected. Competi...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2011-11-29 | E-GEOD-26932 | biostudies-arrayexpress
ProteomicsPull-down of polyP chains and associated proteins with PPBD as a bait
CobB interacts polyP in E. coli stressed cells. The goal of the performed experiment was to identify proteins interacting polyP in E. coli cells encountering amino acid starvation. We used Co-NTA resin with immobilized PPBD for capturing and isolation of polyP, together with bound proteins. The MS a...
ORGANISM(S): Escherichia coli Bacteria 
2026-03-02 | PXD062171 | Pride
Polyp E. coli mg1655 wild-type 
TranscriptomicsTranscription profiling of E. coli grown on mixture of glucose and lactose in a time series
Time series analysis of glucose-lactose diauxie; When cultured on a mixture of glucose and lactose, E. coli grows preferentially on glucose until it is exhausted, resulting in growth arrest while the cells adjust to growth on lactose, i.e., diauxie.
ORGANISM(S): Escherichia coli 
2006-11-27 | E-MEXP-918 | biostudies-arrayexpress
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