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TranscriptomicsSmall RNA pyrosequencing in the protozoan parasite Entamoeba histolytica reveals strain-specific small RNAs that target virulence genes
We provided a full spectrum analysis for E. histolytica AGO2-2 associated 27nt small RNAs. Additionally, comparative analysis of small RNA populations from virulent and non-virulent amebic strains indicates that small RNA populations may regulate virulence genes. AGO2-2 bound small RNAs from E. hist...
ORGANISM(S): Entamoeba histolytica 
2013-01-24 | E-GEOD-43668 | biostudies-arrayexpress
TranscriptomicsGene expression in phagocytic and non-phagocytic Entamoeba histolytica populations
The ability of Entamoeba histolytica to phagocytose host cells correlates to observed virulence in vivo. To better understand the mechanism of phagocytosis we used paramagnetic beads coated with host ligand and sorted trophozoites based on phagocytic ability. Gene expression was then measured in bot...
ORGANISM(S): Entamoeba histolytica 
2012-10-16 | E-GEOD-41604 | biostudies-arrayexpress
ProteomicsComparative Proteomic Study of Entamoeba histolytica Trophozoites, Cysts, and Cyst-Like Structures
Proteomic comparison between trophozoites, cysts and cyst-like structures of Entamoeba histolytica
ORGANISM(S): Entamoeba Histolytica (ncbitaxon:5759) 
2016-04-21 | MSV000079674 | MassIVE
TranscriptomicsRole of REG 1 in Entamoeba histolytica colitis
Differential expression was used to access gene differences after Entamoeba histolytica infection. Entamoeba histolytica is an important diarrheal pathogen worldwide, and induces apoptosis of the intestinal epithelium as part of its disease process. Regenerating (REG) 1 protein is anti-apoptotic. ...
ORGANISM(S): Homo sapiens 
2011-05-24 | E-GEOD-23750 | biostudies-arrayexpress
TranscriptomicsSmall RNAs during E. histolytica stress response
We analyzed ~27nt small RNAs from Entamoeba histolytica trophozoites in basal conditions and after heat shock or oxidative stress E. histolytica trophozoites were treated with 1mM H2O2 for 1hr, or heat shocked at 42°C for 1hr and RNA was isolated and small RNA populations were compared to small RNA ...
ORGANISM(S): Entamoeba histolytica 
2015-07-22 | E-GEOD-67230 | biostudies-arrayexpress
TranscriptomicsEffect of Rom1 silencing on gene expression in Entamoeba histolytica
Entamoeba histolytica trophozoites epigenetically silenced for ameobapore A (G3 parasites) were additionally silenced for the Rhomboid gene Rom1 (EHI_197460) using the mechanism outlined in Mirelman et al. Parasite. 2008 Sep;15(3):266-74 (PMID 18814693). Rom1 silencing was confirmed by RT-PCR. Gene ...
ORGANISM(S): Entamoeba histolytica 
2010-07-07 | E-GEOD-21522 | biostudies-arrayexpress
TranscriptomicsEntamoeba histolytica and auranofin treatment
2 million E. histolytica HM1:IMSS was treated with 0.5% DMSO for 3 h and 2 million E. histolytica HM1:IMSS was treated with 1 M-5M auranofin for 3 h
ORGANISM(S): Entamoeba histolytica 
2012-02-03 | E-MEXP-3494 | biostudies-arrayexpress
TranscriptomicsTargets of the E. histolytica transcription factor URE3-BP
The E. histolytica transcription factor URE3-BP is a calcium-responsive regulator of two E. histolytica virulence genes, hgl5 and fdx1. URE3-BP was previously identified by a yeast one-hybrid screen of E. histolytica proteins capable of binding to the sequence TATTCTATT (URE3) in the promoter region...
ORGANISM(S): Entamoeba histolytica 
2008-10-14 | E-GEOD-12188 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of Entamoeba histolytica trophozoites
This SuperSeries is composed of the following subset Series: GSE2978: Entamoeba heat shock experiments GSE2979: Entamoeba plus Caco2 cells Abstract: We have developed an Entamoeba histolytica genomic DNA microarray and used it to develop a transcriptional profile of 1,971 E. histolytica (HM-1:IMSS)...
ORGANISM(S): Entamoeba histolytica 
2006-03-07 | E-GEOD-4400 | biostudies-arrayexpress
TranscriptomicsEffect of treatment with H2O2 or DPTA-NONOate on E. histolytica
E. histolytica of strains HM1:IMSS (virulent) and Rahman (avirulent) were examined for response to oxidative and nitrosative stress. Response to oxidative stress in E. histolytica was assayed by treating Eh (HM1:IMSS) and Eh (Rahman) with 1mM H2O2 for 60 minutes or 90 minutes and comparing transcri...
ORGANISM(S): Entamoeba histolytica 
2008-09-30 | E-GEOD-11811 | biostudies-arrayexpress
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