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ProteomicsComparison of extracellular matrix enrichment protocols for the improved characterization of the skin matrisome by mass spectrometry
Four protocols were adapted and compared to enrich extracellular matrix proteins from mouse skin: 1. compartmental enrichment; 2. chemical decellularization; 3. enzymatic decellularization using phospholipase A2; 4. quantitative detergent solubility profiling (QDSP). All four protocols were started ...
ORGANISM(S): Mus musculus (Mouse) 
2021-11-02 | PXD025842 | Pride
Ecm enrichment protocols comparison Mouse skin matrisome 
ProteomicsECM characterization of aortas from Adamts5+/+ and Adamts5∆cat AngII-treated mice by LC-MS/MS
Objective - Thoracic aortic aneurysm (TAA), a degenerative disease of the aortic wall, is accompanied by changes in the structure and composition of the aortic extracellular matrix (ECM). The ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family has recently been implicated ...
ORGANISM(S): Mus musculus (Mouse) 
2018-05-08 | PXD009410 | Pride
Aneurysm Protease Ascending aorta Extracellular matrix Lc-ms/ms Adamts 
TranscriptomicsSuppression and Activation of the Malignant Phenotype by Extracellular Matrix in Xenograft Models of Bladder Cancer: A Model for Tumor Cell Dormancy
A major problem in cancer research is the lack of a tractable model for delayed metastasis. Herein we show that cancer cells suppressed by SISgel, a gel-forming normal ECM material derived from Small Intestine Submucosa, in flank xenografts show properties of suppression and re-activation that are v...
ORGANISM(S): Homo sapiens 
2014-02-20 | E-GEOD-35947 | biostudies-arrayexpress
ProteomicsComparison of Protein Expression During Early Phases of Limb Regeneration Between Regeneration-permissive and Regeneration-deficient Axolotl
In this project, we studied the proteomic profiles of the early stages of blastema formation of neotenic and metamorphic axolotls after limb amputation by means of LC-MS/MS technology. We quantified a total of 714 proteins having an adjusted p < 0.01 with FC greater or equal to 2 between two conditi...
ORGANISM(S): Ambystoma mexicanum 
2019-11-25 | PXD014806 | Pride
Proteomics  axolotl  limb regeneration  metamorphosis  gene expression profile 
TranscriptomicsTranscriptomic profiling of LAMA5-deficient human stem cell–derived chondrogenesis reveals a human-specific ECM–WNT signaling axis
This study investigates the transcriptional consequences of Laminin-α5 (LAMA5) loss in human urine-derived stem cells (USCs) to understand the molecular etiology of idiopathic short stature. The experiment consists of two components: 1. Differentiation Profiling: CRISPR/Cas9-mediated LAMA5 knockout...
ORGANISM(S): Homo sapiens 
2026-01-31 | E-MTAB-16566 | biostudies-arrayexpress
TranscriptomicsPre-ovulatory follicle size modulates the periovulatory endocrine environment and endometrial tissue phenotype in cattle
Purpouse: Aims of this study were (1) to characterize the endometrial transcriptome, (2) to identify functional pathways, and (3) to molecularly characterize selected pathways overrepresented in the endometrium of day 7 post-ovulation induction of cows treated to ovulate larger versus smaller follic...
ORGANISM(S): Bos indicus 
2015-07-01 | E-GEOD-65450 | biostudies-arrayexpress
ProteomicsProteomic analysis of extracellular vesicles and conditioned medium from human Adipose-derived Stem/Stromal Cells and Dermal Fibroblasts
Conditioned medium (CM) and extracellular vesicles (EV) from human Adipose-derived Stem/stromal cells (ASC) and Dermal fibroblasts (DF) can represent valid cell substitutes in regenerative medicine and other therapeutic applications. Whether one of the two cell products should be preferred over the ...
ORGANISM(S): Homo sapiens (Human) 
2020-12-23 | PXD020219 | Pride
Adipose-derived stromal cells Label-free quantitative proteomics Extracellular vesicles Dermal fibroblasts Conditioned medium Secretome 
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