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TranscriptomicsEnterohemorrhagic Escherichia coli (EHEC)8624 double deletions of qseC and qseE grown in DMEM
Analysis of effect of deletion of the two adrenergic kinases QseC and QseE on gene expression of EHEC 8624 in the absence and presence of epinephrine A double mutant of qseC and qseE in the EHEC 8624 was grown in the absence or presence of epinephrine to OD600=1.0 in DMEM then processed according to...
ORGANISM(S): Escherichia coli O157:H7 
2012-03-15 | E-GEOD-33895 | biostudies-arrayexpress
TranscriptomicsEnterohemorrhagic Escherichia coli (EHEC) 8624 double deletions of kdpe and cra grown in DMEM
Analysis of effect of deletion of the two transcriptional factors KdpE and Cra on gene expression of EHEC 8624 A double mutant of kdpE and cra in the EHEC 8624 was grown to OD600=1.0 in DMEM then processed according to manufacturer's specifications: http//www.affymetrix.com/support/technical/manual...
ORGANISM(S): Escherichia coli 8624 
2013-07-03 | E-GEOD-43659 | biostudies-arrayexpress
TranscriptomicsEthanolamine gene regulation in EHEC
Bacterial pathogens must be able to both recognize suitable niches within the host for colonization and successfully compete with commensal flora for nutrients in order to establish infection. Ethanolamine (EA) is a major component of mammalian and bacterial membranes and may be used by pathogens as...
ORGANISM(S): Escherichia coli 
2012-06-17 | E-GEOD-34046 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli to investigate bacterial adrenergic signaling
The ability to respond to stress is at the core of an organism’s survival. The hormones epinephrine and norepinephrine play a central role in stress responses in mammals, which require the synchronized interaction of the whole neuroendocrine system. Bacteria also sense and respond to epinephrine a...
ORGANISM(S): Escherichia coli 
2009-11-16 | E-GEOD-15050 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli O157:H7 growth in the absence or presence of HEp-2 epithelial cells
E. coli O157:H7 global gene expression profile is altered during growth in the presence of Hep-2 cells, relative to the absence of cells; We used Affymetrix E. coli 2.0 genechips to determine gene expression changes Experiment Overall Design: E. coli O157:H7 total RNA extracts were collected after a...
ORGANISM(S): Escherichia coli O157:H7 
2009-03-28 | E-GEOD-14238 | biostudies-arrayexpress
TranscriptomicsQseA regulation of virulence factors in EHEC
Enterohemorrhagic E. coli (EHEC) colonizes the large intestine and causes attaching and effacing lesions (AE). Most of the genes involved in the formation of AE lesions are encoded within a chromosomal pathogenicity island termed the Locus of Enterocyte Effacement (LEE). The LysR-like transcriptiona...
ORGANISM(S): Escherichia coli 
2010-09-07 | E-GEOD-18118 | biostudies-arrayexpress
TranscriptomicsHfq virulence regulation in enterohemorhagic Escherichia coli O157:H7 strain 86-24
Escherichia coli O157:H7 is a food-borne pathogen that causes bloody diarrhea and hemolytic uremic syndrome. Hfq is an sRNA chaperone protein that is involved in post-transcriptional regulation of virulence genes in pathogenic bacteria. In EHEC strain EDL933, Hfq acts a negative regulator of the loc...
ORGANISM(S): Escherichia coli O157:H7 
2012-01-06 | E-GEOD-30893 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli O157:H7 w/t LB-Glu 7 hr biofilm cells with various chemicals
E coli O157H7 (EHEC) wildtype 7 hour biofilm cells studied in LB glucose medium with and without chemicals - Epinephrine, Norepinephrine and Indole. Biofilm cells were cultured from glass wool. Experiment Overall Design: Strain: E coli O157H7 (EHEC) Experiment Overall Design: Medium: LB-Glucose Expe...
ORGANISM(S): Escherichia coli 
2008-06-13 | E-GEOD-5552 | biostudies-arrayexpress
TranscriptomicsEHEC and K-12 E. coli QseD mutant analysis
We used arrays to examine the overall transcriptional differences between WT K-12 E. coli, and EHEC 86-24 and their corresponding QseD (yjiE) mutants. Strains were grown to OD600 ~1.0, RNA was harvested, cDNA synthesized, fragmented, labeled and hybridized to the chips. Expression of the QseD mutan...
ORGANISM(S): Escherichia coli 
2010-03-07 | E-GEOD-20413 | biostudies-arrayexpress
TranscriptomicsTranscriptomic analysis for response of EHEC to short chain fatty acids
Enterohaemorrhagic Escherichia coli (EHEC) is an emerging pathogen that causes diarrhea and heamolytic uremic syndrome. Expression of genes associated to pathogenicity is strictly regulated by environmental factors. Since short chian fatty acids (SCFAs) are present in intestinal tract which is a tar...
ORGANISM(S): Escherichia coli 
2010-05-17 | E-GEOD-12903 | biostudies-arrayexpress
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