Hfq virulence regulation in enterohemorhagic Escherichia coli O157:H7 strain 86-24

ABSTRACT: Escherichia coli O157:H7 is a food-borne pathogen that causes bloody diarrhea and hemolytic uremic syndrome. Hfq is an sRNA chaperone protein that is involved in post-transcriptional regulation of virulence genes in pathogenic bacteria. In EHEC strain EDL933, Hfq acts a negative regulator of the locus of enterocyte effacement (LEE) that encodes most of the proteins involved in type three secretion and attaching and effacing lesions. We deleted hfq in E. coli O157:H7 strain 86-24 and compared global transcription profiles of the hfq mutant to the wild type strain in exponential growth phase. Deletion of hfq affected transcription of genes common to nonpathogenic and pathogenic strains of E. coli as well as pathogen-specific genes. Downregulated genes in the hfq mutant included ler as well a

ORGANISM(S): Escherichia coli O157:H7

SUBMITTER: David Rasko 

PROVIDER: E-GEOD-30893 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress