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TranscriptomicsGenome-wide miRNA-expression analysis after p53 activation in colorectal cancer cells
In order to comprehensively identify miRNA-expression changes after p53-activation a miRNA-Seq analysis was conducted after activation of a conditional p53 allele in SW480 cells. SW480/pRTR-p53-VSV cells were subjected to miRNA-Seq analysis after 16 hours doxycycline-treatment. Kompetenzzentrum für ...
ORGANISM(S): Homo sapiens 
2015-07-21 | E-GEOD-67181 | biostudies-arrayexpress
TranscriptomicsAgo-APP: a GW peptide-based Argonaute affinity capture strategy
During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of ...
ORGANISM(S): Arabidopsis thaliana 
2015-09-08 | E-GEOD-70553 | biostudies-arrayexpress
Proteomics,Multiomicsp53-regulated networks of protein, mRNA, miRNA and lncRNA expression revealed by integrated pSILAC and NGS analyses
We determined the effect of p53 activation on de novo protein synthesis using quantitative proteomics of newly synthesized proteins (pulsed stable isotope labeling with amino acids in cell culture, pSILAC) in combination with mRNA and non-coding RNA expression analyses by next generation sequencing ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-05-24 | MSV000079767 | MassIVE
TranscriptomicsNext generation sequencing analysis of microRNAs in brown preadipocytes and in vitro differentiated mature brown fat cells
We performed a genome-wide deep sequencing analysis of the microRNAs abundant in mesenchymal stem cells (MSCs) derived from murine brown adipose tissue and in in vitro differentiated mature brown adipocytes. Several microRNAs were identified as differentially regulated when comparing datasets from M...
ORGANISM(S): Mus musculus 
2013-05-28 | E-GEOD-45499 | biostudies-arrayexpress
Otherp53-regulated networks of protein, mRNA, miRNA and lncRNA expression revealed by integrated pSILAC and NGS analyses.
Data from ProteomeXchange, PXD ID: PXD001976. File: ELITE-RSLC001392.mzml. Published as part of . From the Abstract: {{i}} We determined the effect of p53 activation on de novo protein synthesis using quantitative proteomics of newly synthesized proteins (pulsed stable isotope labeling with amino a...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32320017018 | GPMDB
Resource Reanalysis 
TranscriptomicsCharacterization of the small RNAs of Volvox carteri
The green alga Volvox carteri is a model organism for the development of multicellularity. It has a spherical shape with a complete division of labor between around 2000 somatic cells and 16 reproductive cells. When comparing Volvox with its unicellular relative Chlamydomonas rheinhardtii, one strik...
ORGANISM(S): Volvox carteri 
2016-07-01 | E-GEOD-58704 | biostudies-arrayexpress
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