Ontology highlight
ABSTRACT:
INSTRUMENT(S): Instrument
ORGANISM(S): Homo_sapiens_viruses, Human
DISEASE(S): Not Available
SUBMITTER: Huenten S, et al.
PROVIDER: GPM32320017018 | GPMDB |
REPOSITORIES: GPMDB
Molecular & cellular proteomics : MCP 20150716 10
We determined the effect of p53 activation on de novo protein synthesis using quantitative proteomics (pulsed stable isotope labeling with amino acids in cell culture/pSILAC) in the colorectal cancer cell line SW480. This was combined with mRNA and noncoding RNA expression analyses by next generation sequencing (RNA-, miR-Seq). Furthermore, genome-wide DNA binding of p53 was analyzed by chromatin-immunoprecipitation (ChIP-Seq). Thereby, we identified differentially regulated proteins (542 up, 56 ...[more]