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ProteomicsHuman pluripotent stem cell differentiation into hepatocyte-like cells in microfluidics: secretome and intracellular proteome.
Human pluripotent stem cells (both embryonic and induced pluripotent by reprogramming) were differentiated into hepatic-like cells inside microfluidic culture chambers and in conventional culture systems. Conditioned media and cell lysates were harvested and analyzed. Before starting the differentia...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-07-24 | MSV000084128 | MassIVE
ProteomicsExtracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture
Porcine (Sus scrofa domesticus) small intestinal mucosal layers from the ‘Pietrain’ breed were used. To harvest the intestine, one end was cut in situ and the internal tube was pulled out leaving behind the external layer and mesentery. The retrieved mucosal tissue is then extensively cleaned with d...
ORGANISM(S): Sus Scrofa (ncbitaxon:9823) 
2019-07-26 | MSV000084136 | MassIVE
ProteomicsCellular population dynamics shape the route to human pluripotency.
Each TMT6 sample contains one replicate with the sub-samples representing subsequent time points. Detailed culture and processing methods are described in Panariello et al, Nat Comm, under revision.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-12-22 | MSV000090954 | MassIVE
TranscriptomicsConfined 3D microenvironment regulates early differentiation in Human Pluripotent Stem Cells
We aimed to investigate how different three-dimensional microenvironments regulate the early differentiation of the three germ layers in human embryonic stem cells derived embryoid bodies. In particular, a permeable, biocompatible, hydrogel microwell array was specifically designed for recreating a ...
ORGANISM(S): Homo sapiens 
2012-06-11 | E-GEOD-36480 | biostudies-arrayexpress
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