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RNA abundance decreases linearly from the 5′ to 3′ end of long introns to create “saw-tooth” patterns, and these can be used to infer locations of major splicing events. FUS binds across entire pre-mRNAs with limited sequence specificity, permitting examination of these saw-tooth patterns. We used F...
ORGANISM(S): Homo sapiens 
Brain samples for this dataset were provided by the Medical Research Council Sudden Death Brain and Tissue Bank (Edinburgh, UK). All four individuals sampled were of European descent, neurologically normal during life and confirmed to be neuropathologically normal by a consultant neuropathologist...
Recent studies have shown that expression of many genes with exceptionally long introns in the mammalian brain can be perturbed by regulatory factors linked to neurodevelopmental or neurodegenerative disorders1-3, suggesting unique regulatory mechanisms. Here we identify functional recursive splice ...
we utilize Nano-MeDIP-seq for the analysis of the LT-HSC methylome and, for the first time, simultaneously interrogate the methylome and transcriptome of a homogeneous population of primary murine HSCs, in order to define the underlying causes of changes in HSC functionality during normal ageing. We...
ORGANISM(S): Mus musculus 
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