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MetabolomicsEnzyme engineering for optimizing biosynthesis of 2,4-dihydroxybutyric acid via the synthetic threose-dependent glycolaldehyde assimilation (STEGA) pathway
Ethylene glycol is a potential feedstock for next-generation biorefineries, as it can be derived from both plastic waste and carbon dioxide. The synthetic D-threose-dependent glycolaldehyde assimilation (STEGA) pathway, which is orthogonal to central metabolism of Escherichia coli, was recently cons...
2026-07-15 | MTBLS15050 | MetaboLights
MetabolomicsSAR11 Cells Rely on Enzyme Multifunctionality to Transport and Metabolize a Range of Polyamine Compounds
In the ocean surface layer and cell culture, the polyamine transport protein PotD of SAR11 bacteria is often one of the most abundant proteins detected. Polyamines are organic cations at seawater pH produced by all living organisms and are thought to be an important component of dissolved organic ma...
2021-09-09 | MTBLS3146 | MetaboLights
UnknownEngineering heterologous enzyme secretion in Yarrowia lipolytica.
Not available
S-EPMC9252082 | biostudies-literature
GenomicsSimultaneous in vivo enzyme and host engineering through laboratory evolution
Simultaneous in vivo enzyme and host engineering through laboratory evolution
PRJEB26704 | ENA
ProteomicsCytoplasmic capping enzyme interactome
Formaldehyde crosslinking and Proximity-dependent biotinylation methods were applied to study the cytoplasmic capping enzyme interactome via 1D-LC-MS/MS. The raw data were searched against Uniprot human database modified to contain our designed protein sequences using the Thermo Proteome Discoverer ...
ORGANISM(S): Homo Sapiens (human) 
2018-08-30 | PXD010368 | JPOST Repository
UnknownEnzyme Engineering Database (EnzEngDB): a platform for sharing and interpreting sequence-function relationships across protein engineering campaigns.
Not available
S-EPMC12807667 | biostudies-literature
MetabolomicsMetabolomics analysis of the toxic effects of the production of lycopene and its precursors
Using cells as microbial factories enables highly specific production of chemicals with many advantages over chemical syntheses. A number of exciting new applications of this approach are in the area of precision metabolic engineering, which focuses on improving the specificity of target production....
2021-02-11 | MTBLS642 | MetaboLights
UnknownEngineering enzyme activity using an expanded amino acid alphabet.
Not available
S-EPMC9863031 | biostudies-literature
GenomicsEngineering of novel DNA polymerase variants for single enzyme quantitative multiplex Reverse Transcription-PCR
Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex Reverse Transcription-PCR
PRJNA1176519 | ENA
UnknownAccelerated enzyme engineering by machine-learning guided cell-free expression.
Not available
S-EPMC11747319 | biostudies-literature
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