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Most transgenic crops are produced through tissue culture. The impact of utilizing such methods on the plant epigenome is poorly understood. Here we generated whole-genome, single-nucleotide resolution maps of DNA methylation in several transgenic rice lines. We found that all tested transgenic plan...
ORGANISM(S): Oryza sativa Japonica Group 
Long-term perturbation of de novo chromatin assembly during DNA replication has profound effects on epigenome maintenance and cell fate. The early mechanistic origin of these functional defects is unknown. Here, we combine acute degradation of Chromatin Assembly Factor 1 (CAF-1), a key player in de ...
ORGANISM(S): Homo sapiens (Human) 
2024-11-14 | PXD050618 | Pride
Integrated breeding strategies are used to increase both the yield potential and stability of crops. Most of these approaches have a direct genetic basis. The utility of epigenetics in breeding to improve complex traits such as yield and stress tolerance is not clear. A better understanding of th...
ORGANISM(S): Brassica napus 
Integrated breeding strategies are used to increase both the yield potential and stability of crops. Most of these approaches have a direct genetic basis. The utility of epigenetics in breeding to improve complex traits such as yield and stress tolerance is not clear. A better understanding of th...
ORGANISM(S): Brassica napus 
Linkage-specific ubiquitin chains govern the outcome of numerous critical ubiquitin-dependent signaling processes. However, the targets and functional impacts of linkage-specific ubiquitin modifications remain incompletely understood, due to a paucity of tools for their specific detection and manipu...
ORGANISM(S): Homo sapiens (Human) 
2025-10-21 | PXD056564 | Pride
We have combined standard micrococcal (MNase) digestion of nuclei with a modified protocol for construction paired-end DNA sequencing libraries to map both nucleosomes and subnucleosome-sized particles at single base-pair resolution throughout the budding yeast genome. We found that partially unwrap...
ORGANISM(S): Saccharomyces cerevisiae 
Maintenance of CG methylation (mCG) patterns is essential for chromatin-mediated epigenetic regulation of transcription in plants and mammals. Using successive generations of an Arabidopsis thaliana mutant deficient in maintaining mCG, we found that mCG loss triggered genome-wide activation of alter...
ORGANISM(S): Arabidopsis thaliana 
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