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ProteomicsMass Spectrometry-Driven Epitope Mapping: Application of Diethylpyrocarbonate Covalent Labeling for the Immunotherapeutic Target Programmed Cell Death Protein 1
Monoclonal antibodies (mAbs) have transformed cancer immunotherapy, with anti-PD1 mAbs playing a pivotal role. This study introduces diethylpyrocarbonate covalent labeling-mass spectrometry (DEPC CL-MS) as a rapid and sensitive technique for epitope mapping of PD1, a critical target in mAb developme...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-11-26 | MSV000096536 | MassIVE
ProteomicsIntact Transition Epitope Mapping – Serological Inspection by Epitope EXtraction (ITEM – SIX)
Precision medicine requests accurate serological inspections to precisely stratify patients for targeted treatment. Intact transition epitope mapping analysis proved seroconversion of a model organism's serum when spiked with a monoclonal murine anti-ovalbumin antibody with epitope-resolution. Isola...
ORGANISM(S): Gallus gallus (Chicken) 
2023-03-31 | PXD040754 | Pride
Epitope mapping Item-six Blood serum Seroconversion Offline nanoesi-ms Immune complex analysis 
ProteomicsIntact Transition Epitope Mapping- Thermodynamic Weak-force Order (ITEM-TWO)
Most important characteristics of antibodies are that they typically strongly bind to specific epitopes, thereby expressing low dissociation constants (KDs) and high Gibbs free binding energies (delta Gs). In this study, we describe an off-line nano-electrospray mass spectrometry method, termed IT...
ORGANISM(S): Homo sapiens (Human) 
2019-11-07 | PXD016024 | Pride
Antibody affinity determination Antibody specificity determination 
ProteomicsIntact Transition Epitope Mapping-Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE)
ITEM-THREE is a novel mass spectrometry-based epitope mapping method. To perform ITEM-THREE, antibody-epitope peptide immune-complexes are first generated in electrospray-compatible solutions in which the antibodies maintain their in-solution functionality, by adding an antibody of interest to a mi...
ORGANISM(S): Homo sapiens (Human) 
2019-06-03 | PXD013823 | Pride
Human Molecular recognition Nanoesi Recombinant proteins Ion mobility separation Item-three Collision induced dissociation Epitope antibody complexes 
ProteomicsEpitope mapping of an anti-plasmodium pfMSP119 antibody by Intact Transition Epitope Mapping - Targeted High-Energy Rupture of Extracted Epitopes
Rapid diagnostic tests are first line assays for diagnosing infectious diseases, such as malaria. To minimize false positive and false negative test results in population screening assays, high quality reagents and well characterized antigens and antibodies are needed. An important property of ant...
ORGANISM(S): Homo sapiens (Human) 
2020-08-24 | PXD019717 | Pride
Plasmodium falciparum Epitope mapping Assembled epitope Malaria screening Pfmsp119 antigen 
ProteomicsThe Trypanosoma cruzi Antigen and Epitope Atlas: discovery of antibody specificities in human Chagas Disease patients across human populations
These assays represent an antigen discovery screening, and epitope mapping characterization. In this screening two complete proteomes from Trypanosoma cruzi, from two different strains (CL-Brener, Sylvio X10), were displayed in the form of short peptides (tiling array, overlapped) and assayed with p...
ORGANISM(S): Homo sapiens 
2022-08-20 | E-MTAB-11651 | biostudies-arrayexpress
ProteomicsEpitope Mapping of potential novel immunogenic proteins of Klebsiella pneumoniae MGH 78578
A number of six proteins were selected during immunoscreening and further analyses. The proteins were divided in silico into overlapping 15-mer oligopeptides with an overlap of 11 residues. The microarrays were incubated with different antibodies to K. pneumoniae, C. jejuni and S. aureus. Each micro...
ORGANISM(S): Klebsiella pneumoniae 
2014-02-02 | E-GEOD-52537 | biostudies-arrayexpress
ProteomicsEpitope mapping of potential novel immunogenic proteins of Campylobacter jejuni NCTC 11168
A number of seven proteins were selected during immunoscreening and further analyses. The proteins were in silico divided into overlapping 15-mer oligopeptides with an overlap of 11 residues. The microarrays were incubated with different antibodies to C. jejuni, Escherichia coli and Salmonella enter...
ORGANISM(S): Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 
2013-04-06 | E-GEOD-45542 | biostudies-arrayexpress
ProteomicsThe Trypanosoma cruzi Antigen and Epitope Atlas: fine characterization of antibody specificities in human Chagas Disease patients across human populations
These assays represent antibody-binding assays to analyze seroprevalence of antigens and epitopes at the individual level and also to perform fine epitope mapping characterizations. In this screening 392,299 peptides derived from reactive (antigenic) regions of Trypanosoma cruzi proteins were displa...
ORGANISM(S): Homo sapiens 
2022-08-20 | E-MTAB-11655 | biostudies-arrayexpress
ProteomicsIntact Transition Epitope Mapping - Force differences between Original and Unusual Residues (ITEM-FOUR)
Human cardiac Troponin I epitope peptides were incubated with monoclonal anti-cardiac Troponin I antibody. Immune complexes were subjected to nano electrospray mass spectrometry in order to determine apparent binding energies and dissociation constants of immune complex dissociation reactions in the...
ORGANISM(S): Homo sapiens (Human) 
2023-01-17 | PXD038365 | Pride
Item-four Troponin i Offline nanoesi-ms 
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