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no omics definedMicroRNA profiling by array of human MCF-7 breast cancer cells with ER-beta tagged at the C-terminal or N-terminal and treated with 17-beta-estradiol
Human estrogen-responsive breast cancer cell line MCF-7 TET Off (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha) as previously described.(...
ORGANISM(S): Homo sapiens 
2011-07-29 | E-TABM-1055 | biostudies-arrayexpress
ProteomicsThe role of RNF149 in the pre-emptive quality control substrate ubiquitination
Protein quality control is a continuous process in which a protein's folding status is constantly monitored. Mislocalized proteins (MLP), are processed by the various quality control pathways, as they are often misfolded due to inappropriate cellular surroundings. Polypeptides that fail to transloca...
ORGANISM(S): Homo sapiens (Human) 
2023-04-09 | PXD038478 | Pride
Pre-emptive er associated quality control Membrane translocation Proteasome Rnf149 
TranscriptomicsTranscription profiling by array of human MCF-7 breast cell clones expressing ER-beta tagged with TAP-tag at the C-terminal or N-terminal
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha) as previously described.Cells were cultured in standard growth condit...
ORGANISM(S): Homo sapiens 
2011-10-11 | E-TABM-1053 | biostudies-arrayexpress
TranscriptomicsThe genetic architecture of the genome-wide transcriptional response to ER stress in the mouse
Endoplasmic reticulum (ER) stress occurs when misfolded proteins accumulate in the ER. The cellular response to ER stress involves complex transcriptional and translational changes, important to the survival of the cell. ER stress is a primary cause and a modifier of many human diseases. A first ste...
ORGANISM(S): Mus musculus 
2014-12-02 | E-GEOD-63756 | biostudies-arrayexpress
GenomicsChIP-Seq of human MCF-7 cells with ER-alpha and ER-beta anitbodies following estrogen treatment
We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP...
ORGANISM(S): Homo sapiens 
2010-12-01 | E-MTAB-345 | biostudies-arrayexpress
ProteomicsGPI glycan analysis of GPI-anchored protein from yeast
Quality controlled lipid-based protein sorting into selective ER exit sites
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2022-03-28 | PXD032852 | JPOST Repository
GenomicsAdrenergic receptor signaling orchestrates ER quality control
Selective autophagy of the endoplasmic reticulum (ER-phagy) is essential for maintaining ER proteostasis and cellular fitness, yet the upstream signaling pathways that selectively activate ER turnover remain poorly defined. Here, we identify β2-adren
PRJEB123602 | ENA
TranscriptomicsTranscription profiling by array of human MCF-7 cells with ER-beta tagged with TAP-tag at the C-term or N-term or expressing ER-alpha tagged
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag respectively at the C-term and at the N-term (C-TAP-ER-beta and N-TAP-ER-beta) or expressing ER-alpha tagged (C-TAP-ER-alpha). All were grown in Dulbecco's modified Eagle's medium (DMEM). Then c...
ORGANISM(S): Homo sapiens 
2011-10-11 | E-TABM-1052 | biostudies-arrayexpress
ProteomicsCharacterization of Endoplasmic Reticulum-Associated Degradation in the human fungal pathogen C. albicans
C. albicans is the most prevalent human fungal pathogen. In immunocompromised individuals, C. albicans can cause serious systemic disease and patients infected with drug-resistant isolates have few treatment options. The Ubiquitin-Proteasome System has not been thoroughly characterized in C. albica...
ORGANISM(S): Candida Albicans (ncbitaxon:5476) 
2023-05-03 | MSV000091843 | MassIVE
ProteomicsOligosaccharyltransferase is involved in targeting to ER-associated degradation
The majority of secretory proteins undergo N-glycosylation, a process catalyzed by oligosaccharyltransferase (OST), a membrane-bound protein complex that associates with the translocation channels within the endoplasmic reticulum (ER). Proteins failing quality control undergo ER-associated degradati...
ORGANISM(S): Homo sapiens (Human) 
2025-10-14 | PXD067654 | Pride
Oligosaccharyltransferase Er-associated degradation Protein quality control Protein--folding 
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