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MetabolomicsMetabolomic analysis of riboswitch containing E. coli recombinant expression system
In this study we have employed metabolomics approaches to understand the metabolic effects of producing enhanced green fluorescent protein (eGFP) as a recombinant protein in Escherichia coli cells. This metabolic burden analysis was performed against a number of recombinant expression systems and co...
2017-11-16 | MTBLS393 | MetaboLights
TranscriptomicsGenome-wide characterization of PhoB binding profile in Escherichia coli
This SuperSeries is composed of the following subset Series: GSE21820: Genome-wide characterization of PhoB binding profile in Escherichia coli (gene expression data) GSE21856: Genome-wide characterization of PhoB binding profile in Escherichia coli (ChIP-chip data) Refer to individual Series
ORGANISM(S): Escherichia coli 
2012-10-21 | E-GEOD-21857 | biostudies-arrayexpress
TranscriptomicsE. coli BW25113 ompA mutant vs wild type biofilm and E. coli BW25113 ompA/pCA24N_ompA vs ompA/pCA24N suspension cells
This Series involves two studies: 1) The gene expression of E. coli K-12 BW25113 ompA mutant strain vs. wild type strain glasswool biofilm cells and E. coli K-12 BW25113 ompA mutant vs. wild type polystyrene biofilm cells. 2) The gene expression of E. coli BW25113 ompA/pCA24N_ompA vs. ompA/pCA24N su...
ORGANISM(S): Escherichia coli 
2010-11-03 | E-GEOD-14064 | biostudies-arrayexpress
TranscriptomicsGenome-wide characterization of PhoB binding profile in Escherichia coli (ChIP-chip data)
Chromatin immunoprecipitation was combined with high-density tiling array (ChIP-chip) and gene expression microarray to reveal the adaptive responses of Escherichia coli to phosphate starvation. The first sketch of the genome-wide distribution of PhoB binding profile was unveiled and 43 regions were...
ORGANISM(S): Escherichia coli 
2012-10-21 | E-GEOD-21856 | biostudies-arrayexpress
TranscriptomicsGenome-wide characterization of PhoB binding profile in Escherichia coli (gene expression data)
Chromatin immunoprecipitation was combined with high-density tiling array (ChIP-chip) and gene expression microarray to reveal the adaptive responses of Escherichia coli to phosphate starvation. The first sketch of the genome-wide distribution of PhoB binding profile was unveiled and 43 regions were...
ORGANISM(S): Escherichia coli 
2012-10-21 | E-GEOD-21820 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of E. coli BL21(DE3)::TN7T7 -SOD to analyze the cellular response of this plasmid-free expression system to high level expression of recombinant human super-oxide-dismutase
This transcription profiling time course experiment was conducted in order to analyze the cellular response of the plasmid-free expression system E.coli BL21(DE3)::TN7 to high level expression of recombinant human super-oxide-dismutase (SOD).Three biological replicates were generated by usi...
ORGANISM(S): Escherichia coli 
2013-05-31 | E-MARS-24 | biostudies-arrayexpress
TranscriptomicsChIP-chip of E. coli K-12 MG1655 with antibody against RNA polymerase subunits, RpoB, RpoD, RpoS,RpoH, RpoN, FliA, and FecI
We investigate sigma factor binding regions for each sigma factor under various enviromental and/or genetic conditions. To measure sigma factor binding at a genome scale, we employed a ChIP-chip method to derivative strains of E. coli K-12 MG1655 wild type and its isogenic rpoS and rpoN knock-out s...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2014-02-05 | E-GEOD-46541 | biostudies-arrayexpress
TranscriptomicsChIP-chip of MG1655 with antibody against E. coli RNAP beta subunit under various conditions
We integrated RNAP binding regions (RBRs) and mRNA transcript abundance to determine segments of contiguous transcription originating from promoter regions. To measure RBRs at a genome scale, we employed a ChIP-chip method to E. coli K-12 MG1655 grown in the presence or absence of rifampicin under m...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2009-11-03 | E-GEOD-15588 | biostudies-arrayexpress
TranscriptomicsPet-1 Switches Transcriptional Targets Postnatally to Regulate Maturation of Serotonin Neuron Excitability.
Newborn neurons enter an extended maturation stage, during which they acquire excitability characteristics crucial for development of presynaptic and postsynaptic connectivity. In contrast to earlier specification programs, little is known aboutthe regulatory mechanisms that control neuronal maturat...
ORGANISM(S): Mus musculus 
2016-01-08 | E-GEOD-74315 | biostudies-arrayexpress
ProteomicsPost-translationally modified and stop codon readthrough of recombinant Francisella tularensis Universal stress protein in Escherichia coli
Recombinant Francisella tularensis universal stress protein with a C-terminal histidine-tag (rUsp/His6) was expressed in Escherichia coli. Endogenous F. tularensis Usp has a predicted molecular mass of 30 kDa, but rUsp/His6 had an apparent molecular weight of 33 kDa based on Western blot analyses. ...
ORGANISM(S): Francisella tularensis subsp. tularensis 
2024-05-24 | PXD045793 | Pride
Escherichia coli pet expression system Acetylation Francisella tularensis universal stress protein Transglutaminase Polyamination Stop codon readthrough 
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